Rong Zi-Yi, Lei An-Qi, Wu Qiang-Sheng, Srivastava Anoop Kumar, Hashem Abeer, Abd Allah Elsayed Fathi, Kuča Kamil, Yang Tianyuan
College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui, China.
Front Plant Sci. 2023 Mar 17;14:1146182. doi: 10.3389/fpls.2023.1146182. eCollection 2023.
The culturable endophytic fungus has many beneficial effects on plants, but whether and how it affects physiological activities and phosphorus (P) acquisition of tea seedlings at low P levels is unclear. The objective of this study was to analyze the effects of inoculation with . on growth, gas exchange, chlorophyll fluorescence, auxins, cytokinins, P levels, and expressions of two phosphate transporter () genes in leaves of tea ( L. cv. Fudingdabaicha) seedlings grown at 0.5 μM (P) and 50 μM (P) P levels. Sixteen weeks after the inoculation, . colonized roots of tea seedlings, with root fungal colonization rates reaching 62.18% and 81.34% at P and P levels, respectively. Although plant growth behavior, leaf gas exchange, chlorophyll values, nitrogen balance index, and chlorophyll fluorescence parameters of tea seedlings were suppressed at P versus P levels, inoculation of . mitigated the negative effects to some extent, along with more prominent promotion at P levels. . inoculation significantly increased leaf P and indoleacetic acid concentrations at P and P levels and leaf isopentenyladenine, dihydrozeatin, and transzeatin concentrations at P levels, coupled with the reduction of indolebutyric acid at P levels. Inoculation of . up-regulated the relative expression of leaf at P and P levels and at P levels. It is concluded that . promoted P acquisition and growth in tea seedlings under P deficit conditions by increasing cytokinins and indoleacetic acid and and expression.
可培养内生真菌对植物有许多有益影响，但在低磷水平下它是否以及如何影响茶苗的生理活动和磷（P）吸收尚不清楚。本研究的目的是分析接种[内生真菌名称未给出]对生长在0.5 μM（低磷）和50 μM（高磷）磷水平下的茶树（Camellia sinensis L. cv. Fudingdabaicha）幼苗叶片的生长、气体交换、叶绿素荧光、生长素、细胞分裂素、磷水平以及两个磷转运体（[磷转运体基因名称未给出]）基因表达的影响。接种16周后，[内生真菌名称未给出]定殖于茶苗根系，在低磷和高磷水平下根系真菌定殖率分别达到62.18%和81.34%。尽管与高磷水平相比，低磷水平下茶苗的植物生长行为、叶片气体交换、叶绿素值、氮平衡指数和叶绿素荧光参数受到抑制，但接种[内生真菌名称未给出]在一定程度上减轻了负面影响，在低磷水平下促进作用更显著。接种[内生真菌名称未给出]在低磷和高磷水平下显著提高了叶片磷和吲哚乙酸浓度，在高磷水平下提高了叶片异戊烯腺嘌呤、二氢玉米素和反式玉米素浓度，同时在低磷水平下降低了吲哚丁酸浓度。接种[内生真菌名称未给出]在低磷和高磷水平下上调了叶片[磷转运体基因名称未给出]的相对表达，在高磷水平下上调了[另一个磷转运体基因名称未给出]的相对表达。得出的结论是，[内生真菌名称未给出]通过增加细胞分裂素、吲哚乙酸以及[磷转运体基因名称未给出]和[另一个磷转运体基因名称未给出]的表达，促进了低磷条件下茶苗的磷吸收和生长。
