Malik Muneeb Ahmad, Jan Yasmeena, Al-Keridis Lamya Ahmed, Haq Afrozul, Ahmad Javed, Adnan Mohd, Alshammari Nawaf, Ashraf Syed Amir, Panda Bibhu Prasad
Department of Food Technology, School of Interdisciplinary Science and Technology, Jamia Hamdard, New Delhi 110062, India.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
J Fungi (Basel). 2022 Aug 17;8(8):864. doi: 10.3390/jof8080864.
Vitamin D deficiency is highly prevalent in India and worldwide. Mushrooms are important nutritional foods, and in this context shiitake (Lentinula edodes), button (Agaricus bisporus) and oyster (Pleurotus ostreatus) mushrooms are known for their bioactive properties. The application of ultraviolet (UV) irradiation for the production of substantial amounts of vitamin D2 is well established. Levels of serum 25-hydroxy vitamin D (25-OHD), parathyroid hormone (PTH), calcium, phosphorus and alkaline phosphatase (ALP) were significantly (p < 0.05) improved in vitamin-D-deficient rats after feeding with UVB irradiated mushrooms for 4 weeks. Further, microscopic observations indicate an improvement in the osteoid area and the reduction in trabecular separation of the femur bone. In addition, the level of expression of the vitamin D receptor (VDR) gene and genes metabolizing vitamin D were explored. It was observed that in mushroom-fed and vitamin-D-supplemented groups, there was upregulation of CYP2R1 and VDR, while there was downregulation of CYP27B1 in the liver. Further, CYP2R1 was downregulated, while CYP27B1 and VDR were upregulated in kidney tissue.
维生素D缺乏在印度乃至全球都极为普遍。蘑菇是重要的营养食品，在这方面，香菇（香菇属）、平菇（双孢蘑菇）和牡蛎菇（糙皮侧耳）因其生物活性特性而闻名。紫外线（UV）照射用于大量生产维生素D2的应用已得到充分证实。用紫外线B照射的蘑菇喂养维生素D缺乏的大鼠4周后，血清25-羟基维生素D（25-OHD）、甲状旁腺激素（PTH）、钙、磷和碱性磷酸酶（ALP）水平显著（p<0.05）改善。此外，显微镜观察表明类骨质面积有所改善，股骨小梁间距减小。此外，还探究了维生素D受体（VDR）基因和代谢维生素D的基因的表达水平。观察到，在喂食蘑菇和补充维生素D的组中，肝脏中CYP2R1和VDR上调，而CYP27B1下调。此外，肾组织中CYP2R1下调，而CYP27B1和VDR上调。
