Zelikman Helena, Slutzkey Gil, Rosner Ofir, Levartovsky Shifra, Matalon Shlomo, Beitlitum Ilan
Department of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel.
Department of Periodontology and Dental Implantology, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel.
Materials (Basel). 2022 Aug 18;15(16):5705. doi: 10.3390/ma15165705.
GBR (Guided Bone Regeneration) procedure is challenged by the risk of membrane exposure to the oral cavity and contamination. The barrier quality of these membranes serve as a mechanical block from bacterial penetration into the GBR site. The purpose of this in vitro study was to evaluate the antibacterial effect of three commercial non-resorbable polytetrafluoroethylene membranes. (Two d-PTFE membranes and one double layer e-PTFE +d-PTFE membrane). A validated in vitro model with two bacterial species (Streptococcus sanguinis and Fusobacterium nucleatum) was used. Eight samples from membrane each were placed in a 96-well microtiter plate. The experimental and positive control groups were exposed to a bacterial suspension which involved one bacterial species in each plate. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 h in temperature controlled microplate spectrophotometer under anaerobic conditions. One- Sample Kolmogorov−Smirnov Normal test and the Kruskal−Wallis test was used for the statistical analysis. As shown by the bacterial growth curves obtained from the spectrophotometer readings, all three membranes resulted in bacterial growth. We have not found a statistical difference in F. nucleatum growth between different membrane samples and the positive control group. However, S. sanguinis growth was reduced significantly in the presence of two membranes (CYTOPLAST TXT-200 and NeoGenTM) when compared to the control (p < 0.01). The presence of Permamem® had no significant influence on S. sanguinis growth. Some types of commercial non-resorbable PTFE membranes may have an impact on the growth dynamics of specific bacterial species.
引导骨再生（GBR）手术面临着膜暴露于口腔并受到污染的风险。这些膜的屏障质量起到机械阻挡作用，防止细菌侵入GBR部位。本体外研究的目的是评估三种商用不可吸收聚四氟乙烯膜（两种双层聚四氟乙烯膜和一种双层扩展型聚四氟乙烯+双层聚四氟乙烯膜）的抗菌效果。使用了一个经过验证的包含两种细菌（血链球菌和具核梭杆菌）的体外模型。从每种膜上取8个样本，置于96孔微量滴定板中。实验组和阳性对照组暴露于细菌悬液中，每个平板中包含一种细菌。在厌氧条件下，于温度可控的微量板分光光度计中，在650nm波长下用分光光度法监测细菌生长24小时。采用单样本柯尔莫哥洛夫-斯米尔诺夫正态性检验和克鲁斯卡尔-沃利斯检验进行统计分析。从分光光度计读数获得的细菌生长曲线表明，所有三种膜都导致了细菌生长。我们未发现不同膜样本与阳性对照组之间在具核梭杆菌生长方面存在统计学差异。然而，与对照组相比，在存在两种膜（CYTOPLAST TXT - 200和NeoGenTM）的情况下，血链球菌的生长显著降低（p < 0.01）。Permamem®的存在对血链球菌的生长没有显著影响。某些类型的商用不可吸收聚四氟乙烯膜可能会对特定细菌种类的生长动态产生影响。
