Gil Ana Clara Kuerten, Merino Eugenio A D, Costa Diogo Pontes, Giracca César Nunes, Mazzon Ricardo, Magrin Gabriel Leonardo, de Almeida Josiane, Benfatti Cesar Augusto Magalhães
Department of Implant Dentistry, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil.
Department of Production Engineering, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil.
Dent J (Basel). 2024 Jun 29;12(7):202. doi: 10.3390/dj12070202.
To evaluate, in vitro, the efficiency of a novel apparatus to test the adherence and penetration of bacteria on different membranes for guided regeneration. To create the 3D device, Computer Aided Design/Computer Aided Manufacturing (CAD/CAM) systems were used. Three types of biomaterials were tested ( = 6): (DT) a collagen membrane; (DS) a polymer membrane; and (LP) a dense polytetrafluoroethylene barrier. The biomaterials were adapted to the apparatuses and challenged with two different monospecies bacterial culture of and . After 2 h, bacterial adherence and penetration were quantified by counting the number of colony-forming units (CFUs). Two specimens from each group were used for image analysis using Confocal Laser Scanning Microscopy. Statistical analysis was performed. The DS group had a higher adherence of compared to ( = 0.05). There was less adherence of in the DS group, compared to the LP ( = 0.011) and DT ( < 0.001) groups. Only the membranes allowed penetration, which was blocked by barriers. The DT group allowed a greater penetration of to occur compared to ( = 0.009), which showed a higher penetration into the DS membranes compared to ( = 0.016). The penetration of through DS was higher compared to its penetration through DT and LP ( < 0.01 for both). DT and DS allowed a greater penetration of to occur compared to LP, which prevented both bacterial species from penetrating. The apparatus allowed for the settlement and complete sealing of the biomaterials, enabling standardization.
为了在体外评估一种新型装置测试细菌在不同引导性再生膜上的黏附及穿透效率。使用计算机辅助设计/计算机辅助制造（CAD/CAM）系统制作3D装置。测试了三种生物材料（每组n = 6）：（DT）胶原膜；（DS）聚合物膜；以及（LP）致密聚四氟乙烯屏障。将生物材料适配于装置，并分别用两种不同的单一菌种细菌培养物（金黄色葡萄球菌和大肠杆菌）进行挑战。2小时后，通过计数菌落形成单位（CFU）数量对细菌黏附及穿透进行定量。每组取两个样本用于共聚焦激光扫描显微镜图像分析。进行了统计分析。与大肠杆菌相比，DS组金黄色葡萄球菌的黏附率更高（P = 0.05）。与LP组（P = 0.011）和DT组（P < 0.001）相比，DS组大肠杆菌的黏附较少。只有膜允许穿透，屏障可阻止穿透。与大肠杆菌相比，DT组金黄色葡萄球菌的穿透率更高（P = 0.009），而与金黄色葡萄球菌相比，大肠杆菌在DS膜中的穿透率更高（P = 0.016）。金黄色葡萄球菌通过DS的穿透率高于其通过DT和LP的穿透率（两者P < 0.01）。与LP组相比，DT组和DS组金黄色葡萄球菌和大肠杆菌的穿透率更高，LP组可阻止两种细菌穿透。该装置可实现生物材料的固定和完全密封，从而实现标准化。
