School of Medical Sciences, University of Campinas (UNICAMP), Campinas 13083-888, Brazil.
Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas 13083-871, Brazil.
Molecules. 2022 Aug 9;27(16):5049. doi: 10.3390/molecules27165049.
belongs to the Lamiaceae family, and its constituents show antioxidant, anti-inflammatory, antidepressant, antinociceptive, and antibacterial properties. The aim of this study was to develop a topical formulation with extract that had antimicrobial and antioxidant activity. Maceration, infusion, Soxhlet, and ultrasound were used to produce rosemary extracts, which were submitted to antioxidant, compound quantification, cell viability, and antimicrobial assays. Infusion and Soxhlet showed better results in the DPPH assay. During compound quantification, infusion showed promising metabolite extraction in phenolic compounds and tannins, although maceration was able to extract more flavonoids. The infusion and ultrasound extracts affected more strains of skin bacteria in the disk diffusion assays. In the minimum inhibitory concentration assay, the infusion extract showed results against , and , while ultrasound showed effects against those three bacteria and . The infusion extract was chosen to be incorporated into a green emulsion. The infusion extract promoted lower spreadability and appropriated the texture, and the blank formulation showed high levels of acceptance among the volunteers. According to the results, the rosemary extract showed promising antioxidant and antimicrobial activity, and the developed formulations containing this extract were stable for over 90 days and had acceptable characteristics, suggesting its potential use as a phytocosmetic. This paper reports the first attempt to produce an oil-in-water emulsion using only natural excipients and rosemary extract, which is a promising novelty, as similar products cannot be found on the market or in the scientific literature.
属于唇形科，其成分具有抗氧化、抗炎、抗抑郁、抗伤害和抗菌特性。本研究旨在开发一种具有抗氧化和抗菌活性的含有迷迭香提取物的局部制剂。采用浸渍法、浸提法、索氏提取法和超声提取法制备迷迭香提取物，然后对其进行抗氧化、化合物定量、细胞活力和抗菌试验。浸提法和索氏提取法在 DPPH 试验中表现出更好的效果。在化合物定量分析中，浸提法显示出对酚类化合物和单宁类化合物有更好的提取效果，而浸渍法则能提取更多的类黄酮。浸提法和超声提取法在抑菌环试验中对更多种皮肤细菌菌株有影响。在最低抑菌浓度试验中，浸提法对 、 和 表现出抑菌效果，而超声提取法对这三种细菌都有抑制效果。选择浸提法制备的迷迭香提取物加入到绿色乳剂中。浸提法制备的乳剂具有较低的铺展性和合适的质地，空白制剂在志愿者中表现出较高的接受度。结果表明，迷迭香提取物具有良好的抗氧化和抗菌活性，所开发的含有该提取物的制剂在 90 天以上保持稳定，且具有可接受的特性，提示其作为植物化妆品具有潜在的应用价值。本文首次尝试使用天然赋形剂和迷迭香提取物生产油包水乳液，这是一个很有前景的创新，因为在市场或科学文献中都找不到类似的产品。
