Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Human High Performance & Health Promotion Research Institute: HHP&HP Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.
Molecules. 2022 Aug 20;27(16):5319. doi: 10.3390/molecules27165319.
L. or tamarind seed is an industrial by-product of interest to be investigated for its potential and value-added application. An ethanolic tamarind seed coat (TS) extract was prepared using the maceration technique and used to determine the phytochemical composition and bioactivities. The total phenolic and flavonoid contents were determined using colorimetric methods; moreover, chemical constituents were identified and quantified compared to the standard compounds using the HPLC-UV DAD technique. Bioactivities were investigated using various models: antioxidative activity in a DPPH assay model, anti-melanogenesis in B16 melanoma cells, anti-adipogenesis in 3T3-L1 adipocytes, and anti-microbial activity against , , , and using agar disc diffusion and microdilution methods. The results manifested a high content of catechin as a chemical constituent and multiple beneficiary bioactivities of TS extract, including superior antioxidation to ascorbic acid and catechin, comparable anti-melanogenesis to deoxyarbutin, and significant anti-adipogenesis through inhibition of pre-adipocyte differentiation and reduction of lipid and triglyceride accumulation, and a broad spectral anti-microbial activity with a selectively high susceptibility to when compared to 1% Parabens. Conclusively, TS extract has been revealed as a potential bioactive agent as well as an alternative preservative for application in food, cosmetic, and pharmaceutical product development.
罗望子籽是一种工业副产物，具有潜在的增值应用价值，值得研究。采用浸渍法制备罗望子种皮（TS）提取物，并对其进行了植物化学成分和生物活性的测定。采用比色法测定总酚和总黄酮含量；此外，还采用 HPLC-UV DAD 技术对其化学成分进行了鉴定和定量分析，并与标准化合物进行了比较。采用不同模型研究了生物活性：DPPH 法测定抗氧化活性、B16 黑素瘤细胞测定抗黑色素生成活性、3T3-L1 脂肪细胞测定抗脂肪生成活性以及琼脂平板扩散法和微量稀释法测定对 、 、 、 的抗菌活性。结果表明，TS 提取物含有丰富的儿茶素，具有多种有益的生物活性，包括优于抗坏血酸和儿茶素的抗氧化活性、与脱氧熊果苷相当的抗黑色素生成活性、通过抑制前脂肪细胞分化和减少脂质和甘油三酯积累的显著抗脂肪生成活性，以及对 、 、 和 的广谱抗菌活性，与 1%对羟基苯甲酸酯相比，对 具有较高的选择性敏感性。综上所述，TS 提取物具有作为生物活性物质的潜力，也可作为替代防腐剂，应用于食品、化妆品和药品的开发。
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