聚肌胞诱导 RAW264.7 细胞产生的抗炎成分。
Anti-Inflammatory Constituents of on RAW 264.7 Cells Induced by Polyinosinic-Polycytidylic Acid.
机构信息
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.
Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan.
出版信息
Molecules. 2022 Aug 20;27(16):5320. doi: 10.3390/molecules27165320.
is an endemic mushroom in Taiwan. This study was designed to screen anti-inflammatory compounds from the methanolic extract of the mycelium of on nitric oxide (NO) production in RAW 264.7 cells induced by polyinosinic-polycytidylic acid (poly I:C), a synthetic analog of double-stranded RNA (dsRNA) known to be present in viral infection. A combination of bioactivity-guided isolation with an NMR-based identification led to the isolation of 4-acetylantroquinonol B (), along with seven compounds. The structure of new compounds ( and ) was elucidated by spectroscopic experiments, including MS, IR, and NMR analysis. The anti-inflammatory activity of all isolated compounds was assessed at non-cytotoxic concentrations. 4-Acetylantroquinonol B () was the most potent compound against poly I:C-induced NO production in RAW 264.7 cells with an IC value of 0.57 ± 0.06 μM.
是台湾的一种特有蘑菇。本研究旨在从菌丝体的甲醇提取物中筛选具有抗炎活性的化合物，该提取物来自 。以多聚肌苷酸-多聚胞苷酸（poly I:C）作为诱导剂，诱导 RAW 264.7 细胞产生一氧化氮（NO），poly I:C 是一种双链 RNA（dsRNA）的人工合成类似物，存在于病毒感染中。采用基于生物活性的分离方法与基于 NMR 的鉴定方法相结合，从菌丝体的甲醇提取物中分离得到了 4-乙酰安托醌 B（），以及另外 7 种化合物。通过包括 MS、IR 和 NMR 分析在内的光谱实验阐明了新化合物（和）的结构。所有分离得到的化合物均在非细胞毒性浓度下进行抗炎活性评估。4-乙酰安托醌 B（）对 poly I:C 诱导的 RAW 264.7 细胞中 NO 生成的抑制作用最强，IC 值为 0.57±0.06 μM。
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