Liu Lou, Chen Jiale, Chen Yu, Jiang Shiwen, Xu Hanjie, Zhan Huiying, Ren Yongwei, Xu Dexiang, Xu Zhengfeng, Chen Daozhen
Department of Obstetrics, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China.
School of Public Health, Anhui Medical University, Hefei 230001, China.
Pathogens. 2022 Jul 28;11(8):847. doi: 10.3390/pathogens11080847.
In China, premature rupture of membranes (PROM) counts as a major pregnancy complication in China and usually results into adverse pregnancy outcomes. We analysed the vagina microbiome composition using 16S rDNA V3−V4 amplicon sequencing technology, in this prospective study of 441 women in their third trimester of pregnancy. We first divided all subjects into PROM and HC (healthy control) groups, in order to investigate the correlation of vagina microbiome composition and the development of PROM. We found that seven pathogens were higher in the PROM group as compared to the HC group with statistical significance. We also split all subjects into three groups based on Lactobacillus abundance-dominant (Lactobacillus > 90%), intermediate (Lactobacillus 30−90%) and depleted (Lactobacillus < 30%) groups, and explored nine pathogenic genera that were higher in the depleted group than the intermediate and dominant groups having statistical significance. Finally, using integrated analysis and logistics regression modelling, we discovered that Lactobacillus (coeff = −0.09, p = 0.04) was linked to the decreased risk of PROM, while Gardnerella (coeff = 0.04, p = 0.02), Prevotella (coeff = 0.11, p = 0.02), Megasphaera (coeff = 0.04, p = 0.01), Ureaplasma (coeff = 0.004, p = 0.01) and Dialister (coeff = 0.001, p = 0.04) were associated with the increased risk of PROM. Further study on how these pathogens interact with vaginal microbiota and the host would result in a better understanding of PROM development.
在中国，胎膜早破（PROM）是一种主要的妊娠并发症，通常会导致不良妊娠结局。在这项针对441名孕晚期女性的前瞻性研究中，我们使用16S rDNA V3-V4扩增子测序技术分析了阴道微生物群组成。我们首先将所有受试者分为胎膜早破组和健康对照组（HC），以研究阴道微生物群组成与胎膜早破发生之间的相关性。我们发现，与健康对照组相比，胎膜早破组中有7种病原体的含量更高，具有统计学意义。我们还根据乳酸杆菌丰度将所有受试者分为三组，即优势组（乳酸杆菌>90%）、中间组（乳酸杆菌30%-90%）和匮乏组（乳酸杆菌<30%），并探究了9种在匮乏组中含量高于中间组和优势组且具有统计学意义的致病属。最后，通过综合分析和逻辑回归建模，我们发现乳酸杆菌（系数=-0.09，p=0.04）与胎膜早破风险降低有关，而加德纳菌（系数=0.04，p=0.02）、普雷沃菌（系数=0.11，p=0.02）、巨球形菌（系数=0.04，p=0.01）、脲原体（系数=0.004，p=0.01）和小杆菌属（系数=0.001，p=0.04）与胎膜早破风险增加有关。进一步研究这些病原体如何与阴道微生物群和宿主相互作用，将有助于更好地理解胎膜早破的发生机制。
