Quach Hung, Le Tuong-Vi, Nguyen Thanh-Thuy, Nguyen Phuong, Nguyen Cuu Khoa, Dang Le Hang
Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam.
Vietnam Academy of Science and Technology, Graduate University of Science and Technology, Ho Chi Minh City 700000, Vietnam.
Pharmaceutics. 2022 Aug 9;14(8):1654. doi: 10.3390/pharmaceutics14081654.
Lipid nanoparticles based on lecithin are an interesting part of drug delivery systems. However, the stability of lecithin nano-lipids is problematic due to the degradation of lecithin, causing a decrease in pH. In this study, the modification of the conventional nano-lipid-based soybean lecithin was demonstrated. Ginger-oil-derived Zingiber officinale was used along with lecithin, cholesterol and span 80 to fabricate nano-lipids (GL nano-lipids) using a thin-film method. TEM and a confocal microscope were used to elucidate GL nano-lipids' liposome-like morphology. The average size of the resultant nano-lipid was 249.1 nm with monodistribution (PDI = 0.021). The ζ potential of GL nano-lipids was negative, similarly to as-prepared nano-lipid-based lecithin. GL nano-lipid were highly stable over 60 days of storage at room temperature in terms of size and ζ potential. A shift in pH value from alkaline to acid was detected in lecithin nano-lipids, while with the incorporation of ginger oil, the pH value of nano-lipid dispersion was around 7.0. Furthermore, due to the richness of shogaol-6 and other active compounds in ginger oil, the GL nano-lipid was endowed with intrinsic antibacterial activity. In addition, the sulforhodamine B (SRB) assay and live/dead imaging revealed the excellent biocompatibility of GL nano-lipids. Notably, GL nano-lipids were capable of carrying hydrophobic compounds such as curcumin and performed a pH-dependent release profile. A subsequent characterization showed their suitable potential for drug delivery systems.
基于卵磷脂的脂质纳米颗粒是药物递送系统中一个有趣的组成部分。然而，由于卵磷脂的降解导致pH值下降，卵磷脂纳米脂质的稳定性存在问题。在本研究中，展示了对传统的基于纳米脂质的大豆卵磷脂的改性。将姜油来源的姜与卵磷脂、胆固醇和司盘80一起使用，采用薄膜法制备纳米脂质（GL纳米脂质）。使用透射电子显微镜（TEM）和共聚焦显微镜来阐明GL纳米脂质的脂质体样形态。所得纳米脂质的平均尺寸为249.1 nm，呈单分散分布（多分散指数PDI = 0.021）。GL纳米脂质的ζ电位为负，与制备的基于卵磷脂的纳米脂质类似。GL纳米脂质在室温下储存60天期间，在尺寸和ζ电位方面具有高度稳定性。在卵磷脂纳米脂质中检测到pH值从碱性向酸性转变，而加入姜油后，纳米脂质分散体的pH值约为7.0。此外，由于姜油中富含姜辣素-6和其他活性化合物，GL纳米脂质具有内在的抗菌活性。此外，磺酰罗丹明B（SRB）测定和活/死成像显示GL纳米脂质具有优异的生物相容性。值得注意的是，GL纳米脂质能够携带诸如姜黄素等疏水性化合物，并呈现出pH依赖性释放曲线。随后的表征表明它们在药物递送系统中具有合适的潜力。
