Osorio Raquel, Asady Samara, Toledano-Osorio Manuel, Toledano Manuel, Bueno Juan M, Martínez-Ojeda Rosa M, Osorio Estrella
Faculty of Dentistry, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.
Medicina Clínica y Salud Pública Programme, University of Granada, 18071 Granada, Spain.
Polymers (Basel). 2022 Aug 11;14(16):3274. doi: 10.3390/polym14163274.
Natural extracellular matrix (ECM) collagen membranes are frequently used for bone regeneration procedures. Some disadvantages, such as rapid degradation and questionable mechanical properties, limit their clinical use. These membranes have a heterologous origin and may proceed from different tissues. Biomineralization is a process in which hydroxyapatite deposits mainly in collagen fibrils of the matrices. However, when this deposition occurs on the ECM, its mechanical properties are increased, facilitating bone regeneration. The objective of the present research is to ascertain if different membranes from distinct origins may undergo biomineralization. Nanomechanical properties, scanning electron (SEM) and multiphoton (MP) microscopy imaging were performed in three commercially available ECMs before and after immersion in simulated body fluid solution for 7 and 21 d. The matrices coming from porcine dermis increased their nanomechanical properties and they showed considerable mineralization after 21 d, as observed in structural changes detected through SEM and MP microscopy. It is hypothesized that the more abundant crosslinking and the presence of elastin fibers within this membrane explains the encountered favorable behavior.
天然细胞外基质（ECM）胶原膜常用于骨再生手术。然而，一些缺点，如快速降解和机械性能存疑，限制了它们的临床应用。这些膜具有异源起源，可能来自不同组织。生物矿化是一个过程，其中羟基磷灰石主要沉积在基质的胶原纤维中。然而，当这种沉积发生在ECM上时，其机械性能会增强，有利于骨再生。本研究的目的是确定不同来源的膜是否会发生生物矿化。在三种市售的ECM浸入模拟体液溶液7天和21天后，分别进行了纳米力学性能、扫描电子显微镜（SEM）和多光子（MP）显微镜成像分析。来自猪真皮的基质其纳米力学性能有所提高，并且在21天后显示出大量矿化，这通过SEM和MP显微镜检测到的结构变化得以观察到。据推测，该膜中更丰富的交联和弹性纤维的存在解释了所观察到的良好表现。
