Wu Xiao-Ting, Mei May Lei, Li Quan-Li, Cao Chris Ying, Chen Jia-Long, Xia Rong, Zhang Zhi-Hong, Chu Chun Hung
College & Hospital of Stomatology, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China.
Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China.
Materials (Basel). 2015 Nov 20;8(11):7889-7899. doi: 10.3390/ma8115433.
This study aimed to accelerate the remineralization of a completely demineralized dentine collagen block in order to regenerate the dentinal microstructure of calcified collagen fibrils by a novel electric field-aided biomimetic mineralization system in the absence of non-collagenous proteins. Completely demineralized human dentine slices were prepared using ethylene diamine tetraacetic acid (EDTA) and treated with guanidine hydrochloride to extract the bound non-collagenous proteins. The completely demineralized dentine collagen blocks were then remineralized in a calcium chloride agarose hydrogel and a sodium hydrogen phosphate and fluoride agarose hydrogel. This process was accelerated by subjecting the hydrogels to electrophoresis at 20 mA for 4 and 12 h. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM) were used to evaluate the resultant calcification of the dentin collagen matrix. SEM indicated that mineral particles were precipitated on the intertubular dentin collagen matrix; these densely packed crystals mimicked the structure of the original mineralized dentin. However, the dentinal tubules were not occluded by the mineral crystals. XRD and EDX both confirmed that the deposited crystals were fluorinated hydroxyapatite. TEM revealed the existence of intrafibrillar and interfibrillar mineralization of the collagen fibrils. A novel electric field-aided biomimetic mineralization system was successfully developed to remineralize a completely demineralized dentine collagen matrix in the absence of non-collagenous proteins. This study developed an accelerated biomimetic mineralization system which can be a potential protocol for the biomineralization of dentinal defects.
本研究旨在通过一种新型电场辅助仿生矿化系统，在不存在非胶原蛋白的情况下，加速完全脱矿的牙本质胶原块的再矿化，以再生钙化胶原纤维的牙本质微观结构。使用乙二胺四乙酸（EDTA）制备完全脱矿的人牙本质切片，并用盐酸胍处理以提取结合的非胶原蛋白。然后将完全脱矿的牙本质胶原块在氯化钙琼脂糖水凝胶和磷酸氢二钠及氟化物琼脂糖水凝胶中进行再矿化。通过在20 mA下对水凝胶进行4小时和12小时的电泳来加速这一过程。使用X射线衍射（XRD）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）和透射电子显微镜（TEM）来评估所得牙本质胶原基质的钙化情况。SEM表明矿物颗粒沉淀在管间牙本质胶原基质上；这些紧密堆积的晶体模仿了原始矿化牙本质的结构。然而，牙本质小管未被矿物晶体堵塞。XRD和EDX均证实沉积的晶体为氟化羟基磷灰石。TEM揭示了胶原纤维内和纤维间矿化的存在。成功开发了一种新型电场辅助仿生矿化系统，可在不存在非胶原蛋白的情况下使完全脱矿的牙本质胶原基质再矿化。本研究开发了一种加速仿生矿化系统，该系统可能成为牙本质缺损生物矿化的潜在方案。
