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2011年至2018年华南地区日本脑炎病毒分离株的基因组特征及E蛋白生物信息学分析

Genomic Characteristics and E Protein Bioinformatics Analysis of JEV Isolates from South China from 2011 to 2018.

作者信息

Sun Yawei, Ding Hongxing, Zhao Feifan, Yan Quanhui, Li Yuwan, Niu Xinni, Zeng Weijun, Wu Keke, Ling Bing, Fan Shuangqi, Zhao Mingqiu, Yi Lin, Chen Jinding

机构信息

Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.

出版信息

Vaccines (Basel). 2022 Aug 12;10(8):1303. doi: 10.3390/vaccines10081303.

DOI:10.3390/vaccines10081303
PMID:36016192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9412759/
Abstract

Japanese encephalitis is a mosquito-borne zoonotic epidemic caused by the Japanese encephalitis virus (JEV). JEV is not only the leading cause of Asian viral encephalitis, but also one of the leading causes of viral encephalitis worldwide. To understand the genetic evolution and E protein characteristics of JEV, 263 suspected porcine JE samples collected from South China from 2011 to 2018 were inspected. It was found that 78 aborted porcine fetuses were JEV-nucleic-acid-positive, with a positive rate of 29.7%. Furthermore, four JEV variants were isolated from JEV-nucleic-acid-positive materials, namely, CH/GD2011/2011, CH/GD2014/2014, CH/GD2015/2015, and CH/GD2018/2018. The cell culture and virus titer determination of four JEV isolates showed that four JEV isolates could proliferate stably in Vero cells, and the virus titer was as high as 10 TCID 50/mL. The whole-genome sequences of four JEV isolates were sequenced. Based on the phylogenetic analysis of the JEV E gene and whole genome, it was found that CH/GD2011/2011 and CH/GD2015/2015 belonged to the GIII type, while CH/GD2014/2014 and CH/GD2018/2018 belonged to the GI type, which was significantly different from that of the JEV classical strain CH/BJ-1/1995. Bioinformatics tools were used to analyze the E protein phosphorylation site, glycosylation site, B cell antigen epitope, and modeled 3D structures of E protein in four JEV isolates. The analysis of the prevalence of JEV and the biological function of E protein can provide a theoretical basis for the prevention and control of JEV and the design of antiviral drugs.

摘要

日本脑炎是由日本脑炎病毒（JEV）引起的一种蚊媒人畜共患流行病。JEV不仅是亚洲病毒性脑炎的主要病因，也是全球病毒性脑炎的主要病因之一。为了解JEV的基因进化和E蛋白特征，对2011年至2018年从中国南方采集的263份疑似猪日本脑炎样本进行了检测。结果发现，78例流产猪胎儿JEV核酸呈阳性，阳性率为29.7%。此外，从JEV核酸阳性材料中分离出4株JEV变异株，即CH/GD2011/2011、CH/GD2014/2014、CH/GD2015/2015和CH/GD2018/2018。对4株JEV分离株进行细胞培养和病毒滴度测定，结果显示4株JEV分离株均可在Vero细胞中稳定增殖，病毒滴度高达10 TCID50/mL。对4株JEV分离株的全基因组序列进行了测序。基于JEV E基因和全基因组的系统发育分析，发现CH/GD2011/2011和CH/GD2015/2015属于GIII型，而CH/GD2014/2014和CH/GD2018/2018属于GI型，这与JEV经典株CH/BJ-1/1995明显不同。利用生物信息学工具分析了4株JEV分离株E蛋白的磷酸化位点、糖基化位点、B细胞抗原表位，并对E蛋白的三维结构进行了建模。对JEV流行情况和E蛋白生物学功能的分析可为JEV的防控及抗病毒药物的设计提供理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf5e/9412759/e9d1ba46b4e1/vaccines-10-01303-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf5e/9412759/cd98487655ce/vaccines-10-01303-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf5e/9412759/f5fbcac9c091/vaccines-10-01303-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf5e/9412759/1f489611cd0c/vaccines-10-01303-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf5e/9412759/e9d1ba46b4e1/vaccines-10-01303-g010.jpg

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