Laboratory of Biochemistry and Molecular Virology, Department of Molecular Biology and Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece.
Clinical Immunology-Rheumatology Unit, 2nd Department of Medicine and Laboratory, Hippokration General Hospital of Athens, 11527 Athens, Greece.
Viruses. 2022 Jul 31;14(8):1694. doi: 10.3390/v14081694.
Hepatitis C virus is the major cause of chronic liver diseases and the only cytoplasmic RNA virus known to be oncogenic in humans. The viral genome gives rise to ten mature proteins and to additional proteins, which are the products of alternative translation initiation mechanisms. A protein-known as ARFP (alternative reading frame protein) or Core+1 protein-is synthesized by an open reading frame overlapping the HCV Core coding region in the (+1) frame of genotype 1a. Almost 20 years after its discovery, we still know little of the biological role of the ARFP/Core+1 protein. Here, our differential proteomic analysis of stable hepatoma cell lines expressing the Core+1/Long isoform of HCV-1a relates the expression of the Core+1/Long isoform with the progression of the pathology of HCV liver disease to cancer.
丙型肝炎病毒是慢性肝脏疾病的主要病因，也是唯一被证实具有人类致癌性的细胞质 RNA 病毒。该病毒基因组产生 10 种成熟蛋白和其他蛋白，这些蛋白是通过不同的翻译起始机制产生的。一种被称为 ARFP（交替阅读框蛋白）或 Core+1 蛋白的蛋白，是通过 HCV 核心编码区在 1a 基因型的（+1）框架内重叠的开放阅读框合成的。在发现近 20 年后，我们对 ARFP/Core+1 蛋白的生物学作用仍知之甚少。在这里，我们对稳定表达 HCV-1a 的 Core+1/长型的肝癌细胞系进行差异蛋白质组学分析，将 Core+1/长型的表达与 HCV 肝病向癌症的病理进展联系起来。
