Nuffield Department of Medicine, University of Oxford, Oxford OX3 7FZ, UK.
Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Division of Sleep Medicine, Harvard Medical School, Boston, MA 02115, USA.
Viruses. 2022 Aug 5;14(8):1728. doi: 10.3390/v14081728.
Circadian rhythms influence and coordinate an organism's response to its environment and to invading pathogens. We studied the diurnal variation in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in nasal/throat swabs collected in late 2020 to spring 2021 in a population immunologically naïve to SARS-CoV-2 and prior to widespread vaccination. SARS-CoV-2 diagnostic PCR data from 1698 participants showed a significantly higher viral load in samples obtained in the afternoon, in males, and in hospitalised patients when linear mixed modelling was applied. This study illustrates the importance of recording sample collection times when measuring viral replication parameters in clinical and research studies.
昼夜节律会影响并协调生物体对环境和入侵病原体的反应。我们研究了 2020 年末至 2021 年初在对 SARS-CoV-2 无免疫人群中采集的鼻/咽拭子中严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）RNA 的昼夜变化，且这些样本采集均在广泛接种疫苗之前。应用线性混合模型对 1698 名参与者的 SARS-CoV-2 诊断 PCR 数据进行分析，结果显示，下午采集的样本、男性样本和住院患者样本中的病毒载量明显更高。本研究说明了在临床和研究中测量病毒复制参数时记录样本采集时间的重要性。
