Leavitt M C, Ito J
Nucleic Acids Res. 1987 Jul 10;15(13):5251-9. doi: 10.1093/nar/15.13.5251.
The nucleotide sequence of Bacillus phage Nf gene E has been determined. Gene E codes for phage terminal protein which is the primer necessary for the initiation of DNA replication. The deduced amino acid sequence of Nf terminal protein is approximately 66% homologous with the terminal proteins of Bacillus phages PZA and luminal diameter 29, and shows similar hydropathy and secondary structure predictions. A serine which has been identified as the residue which covalently links the protein to the 5' end of the genome in luminal diameter 29, is conserved in all three phages. The hydropathic and secondary structural environment of this serine is similar in these phage terminal proteins and also similar to the linking serine of adenovirus terminal protein.
芽孢杆菌噬菌体Nf基因E的核苷酸序列已被确定。基因E编码噬菌体末端蛋白,该蛋白是DNA复制起始所必需的引物。推导的Nf末端蛋白氨基酸序列与芽孢杆菌噬菌体PZA和29号噬菌体的末端蛋白约66%同源,并且显示出相似的亲水性和二级结构预测。在29号噬菌体中已被鉴定为将该蛋白与基因组5'端共价连接的残基的丝氨酸,在所有三种噬菌体中都是保守的。该丝氨酸的亲水性和二级结构环境在这些噬菌体末端蛋白中相似,并且也类似于腺病毒末端蛋白的连接丝氨酸。