Hamdalla Hadeer Mohamed, Ahmed Rasha Rashad, Galaly Sanaa Rida, Ahmed Osama Mohamed, Naguib Ibrahim A, Alghamdi Badrah S, Abdul-Hamid Manal
Cell Biology, Histology and Genetics Division, Department of Zoology, Faculty of Science, Beni-Suef University, P.O. Box 62521, Beni Suef, Egypt.
Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, P.O. Box 62521, Beni Suef, Egypt.
Stem Cells Int. 2022 Aug 16;2022:1900403. doi: 10.1155/2022/1900403. eCollection 2022.
Osteoarthritis (OA) of the knee is a debilitating condition that can severely limit an individual's mobility and quality of life. This study was designed to evaluate the efficacy of bone marrow-derived mesenchymal stem cell (BM-MSC) treatment in cartilage repair using a rat model of monoiodoacetate- (MIA-) induced knee OA. OA was induced in the knee joint of rats by an intracapsular injection of MIA (2 mg/50 L) on day zero. The rats were divided into three groups ( = 6): a normal control group, an osteoarthritic control group, and an osteoarthritic group receiving a single intra-articular injection of BM-MSCs (5 × 10 cells/rat). The knee diameter was recorded once per week. By the end of the performed experiment, X-ray imaging and enzyme-linked immunosorbent assay analysis of serum inflammatory cytokines interleukin-1beta (IL-), IL-6, and tumor necrosis factor- (TNF-) and anti-inflammatory cytokines interleukin-10 and transforming growth factor-beta (TGF-) were carried out. In addition, RT-PCR was used to measure nuclear factor-kappa B (NF-B), inducible nitric oxide synthase (iNOS), and type II collagen mRNA levels and Western blot analysis was used to determine caspase-3 protein levels in all treated groups. Finally, hematoxylin/and eosin stains were used for histopathological investigation. Administration of BM-MSCs significantly downregulated knee joint swelling and MIA-induced (IL-1, IL-6, and TNF-) and upregulated IL-10 and TGF- as well. Moreover, BM-MSC-treated osteoarthritic rats exhibited decreased expression of NF-B, iNOS, and apoptotic mediator (caspase-3) and increased expression of type II collagen when compared to rats treated with MIA alone. The hematoxylin/eosin-stained sections revealed that BM-MSC administration ameliorated the knee joint alterations in MIA-injected rats. BM-MSCs could be an effective treatment for inflamed knee joints in the MIA-treated rat model of osteoarthritis, and the effect may be mediated via its anti-inflammatory and antioxidant potential.
膝关节骨关节炎（OA）是一种使人衰弱的疾病，会严重限制个体的活动能力和生活质量。本研究旨在使用单碘乙酸盐（MIA）诱导的大鼠膝关节OA模型，评估骨髓间充质干细胞（BM-MSC）治疗对软骨修复的疗效。在第0天通过关节囊内注射MIA（2mg/50μL）在大鼠膝关节诱导OA。将大鼠分为三组（每组n = 6）：正常对照组、骨关节炎对照组和接受单次关节内注射BM-MSCs（5×10⁶细胞/只大鼠）的骨关节炎组。每周记录一次膝关节直径。在进行的实验结束时，进行X线成像以及血清炎性细胞因子白细胞介素-1β（IL-1β）、IL-6和肿瘤坏死因子-α（TNF-α）以及抗炎细胞因子白细胞介素-10和转化生长因子-β（TGF-β）的酶联免疫吸附测定分析。此外，使用逆转录聚合酶链反应（RT-PCR）测量所有治疗组中核因子-κB（NF-κB）、诱导型一氧化氮合酶（iNOS）和II型胶原mRNA水平，并使用蛋白质免疫印迹分析确定caspase-3蛋白水平。最后，使用苏木精和伊红染色进行组织病理学研究。BM-MSCs的给药显著下调膝关节肿胀以及MIA诱导的（IL-1、IL-6和TNF-α）水平，并上调IL-10和TGF-β水平。此外，与单独用MIA治疗的大鼠相比，接受BM-MSC治疗的骨关节炎大鼠表现出NF-κB、iNOS和凋亡介质（caspase-3）表达降低以及II型胶原表达增加。苏木精/伊红染色切片显示，BM-MSCs给药改善了MIA注射大鼠的膝关节改变。在MIA治疗的骨关节炎大鼠模型中，BM-MSCs可能是治疗膝关节炎症的有效方法，其效果可能通过其抗炎和抗氧化潜力介导。
