Department of Clinical Laboratory, The Secondary Hospital of Tianjin Medical University, Tianjin, China.
Department of Pancreatic Cancer, Tianjin Tumor Hospital, Tianjin, China.
Comput Math Methods Med. 2022 Aug 16;2022:6426800. doi: 10.1155/2022/6426800. eCollection 2022.
To assess KIF11 expression in human thyroid tumor tissues and further evaluate its involvement in thyroid cancer.
The expression of KIF11 in 71 cases of thyroid carcinoma as well as corresponding tissues was detected by the immunohistochemical (IHC) method. Patients were divided into the high KIF11 expression as well as low expression groups based on the staining levels. In addition, to study the relationship between the expression of KIF11 as well as clinicopathological features, the effects of KIF11 were detected on the proliferation, apoptosis, and cell cycle of two types of thyroid cancer cells, TPC-1 and KTC-1, through colony formation assays, MTT assays, and FCM assays, respectively. We further assessed the potential effects of KIF11 on tumor growth using an animal model.
The significantly high expression of KIF11 in thyroid tumor tissues was revealed, and the correlations between KIF11 expression levels as well as clinical pathological features (T stage and intraglandular dissemination) of patients were revealed. We further noticed that KIF11 knockdown remarkably suppressed thyroid cancer cell proliferation as well as induced cell apoptosis of thyroid cancer cells. Additionally, KIF11 contributed to tumor growth of thyroid cancer cells in mice.
We noticed the involvement of KIF11 in the progression of thyroid cancer.
评估 KIF11 在人甲状腺肿瘤组织中的表达，并进一步评估其在甲状腺癌中的作用。
采用免疫组织化学（IHC）方法检测 71 例甲状腺癌及相应组织中 KIF11 的表达。根据染色水平将患者分为 KIF11 高表达组和低表达组。此外，为了研究 KIF11 表达与临床病理特征的关系，通过集落形成实验、MTT 实验和 FCM 实验分别检测 KIF11 对两种甲状腺癌细胞 TPC-1 和 KTC-1 的增殖、凋亡和细胞周期的影响。我们还使用动物模型评估了 KIF11 对肿瘤生长的潜在影响。
揭示了 KIF11 在甲状腺肿瘤组织中表达明显升高，并揭示了 KIF11 表达水平与患者临床病理特征（T 分期和腺内播散）之间的相关性。我们进一步注意到，KIF11 敲低显著抑制了甲状腺癌细胞的增殖，并诱导了甲状腺癌细胞的凋亡。此外，KIF11 促进了甲状腺癌细胞在小鼠中的肿瘤生长。
我们注意到 KIF11 参与了甲状腺癌的进展。
