Yin Xuechen, Fan Xiangyi, Zhou Zipeng, Li Qi
College of Basic Medicine, Jinzhou Medical University, Jinzhou, China.
Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Front Chem. 2022 Aug 9;10:964662. doi: 10.3389/fchem.2022.964662. eCollection 2022.
Chronic wound healing in diabetic patients had been considered a major clinical challenge, so there was an urgent need to establish more effective treatment methods. In this study, we prepared berberine-modified ZnO nano-colloids hydrogel (ZnO-Ber/H) and evaluated its wound healing performance in a diabetic rat. The prepared ZnO-Ber/H had excellent moisturizing, anti-inflammatory and anti-oxidative stress abilities. , ZnO-Ber/H could effectively up-regulate antioxidant stress factors (Nrf2, HO-1, NQO1) by 4.65-fold, 2.49-fold, 2.56-fold, respectively. experiments have shown that ZnO-Ber/H could effectively improve the wound healing rate (92.9%) after 15 days of treatment. Meanwhile, the ability of anti-oxidative stress had also been verified . ZnO-Ber/H down-regulated inflammatory factor (TNF-α, IL-1β, and IL-6) by 72.8%, 55% and 71% respectively, up-regulated vascular related factors VEGF and CD31 by 3.9-fold and 3.2-fold by Western blot. At the same time, ZnO-Ber/H could promote the expression of EGFR and FGFR, thereby affecting the generation of new epithelial tissue. Based on extensive characterization and biological evaluation, ZnO-Ber/H was expected to be a potential candidate for promoting diabetic wound healing.
糖尿病患者慢性伤口愈合一直被认为是一项重大临床挑战，因此迫切需要建立更有效的治疗方法。在本研究中，我们制备了黄连素修饰的ZnO纳米胶体水凝胶（ZnO-Ber/H），并评估了其在糖尿病大鼠中的伤口愈合性能。所制备的ZnO-Ber/H具有优异的保湿、抗炎和抗氧化应激能力。ZnO-Ber/H能有效上调抗氧化应激因子（Nrf2、HO-1、NQO1），分别上调4.65倍、2.49倍、2.56倍。实验表明，治疗15天后，ZnO-Ber/H能有效提高伤口愈合率（92.9%）。同时，抗氧化应激能力也得到了验证。通过蛋白质印迹法，ZnO-Ber/H使炎症因子（TNF-α、IL-1β和IL-6）分别下调72.8%、55%和71%，使血管相关因子VEGF和CD31分别上调3.9倍和3.2倍。同时，ZnO-Ber/H可促进EGFR和FGFR的表达，从而影响新上皮组织的生成。基于广泛的表征和生物学评价，ZnO-Ber/H有望成为促进糖尿病伤口愈合的潜在候选物。
