Hippeläinen M, Naukkarinen A, Alhava E, Sorvari T E
Poult Sci. 1987 Mar;66(3):514-20. doi: 10.3382/ps.0660514.
To study the role of the chicken bursa of Fabricius separately from the rest of the gut-associated lymphoid tissue (GALT), four operating methods were developed; these techniques are described in detail. Bursal function and immunological state of the operated chickens were assessed by applying colloidal carbon, Brucella abortus organism, and sheep red blood cells (SRBC) into the isolated bursa and then measuring the humoral immune responses. Closing the bursal duct, after administering antigens through it, was shown to be a reliable and reproducible method for immunizing chickens strictly via the bursal system. In this method, morphology and endocytosis of carbon in the isolated bursae were normal. Furthermore, the anti-Brucella titers did not significantly differ from those in the unoperated per anum immunized control chickens, whereas the response to SRBC was significantly lower (P less than .001) in the operated chickens compared with controls. Separation of the bursa from the rest of the GALT showed that the chicken bursa of Fabricius functions as a peripheral lymphoid organ; specific antibodies are formed against antigens introduced into the bursal lumen. The operating method also has confirmed that Brucella is a bursa-dependent antigen, but that SRBC need cooperation of the GALT to evolve a good humoral immune response.
为了单独研究鸡法氏囊在肠道相关淋巴组织（GALT）其余部分之外的作用，开发了四种手术方法；这些技术将详细描述。通过将胶体碳、流产布鲁氏菌和绵羊红细胞（SRBC）注入分离出的法氏囊中，然后测量体液免疫反应，来评估手术鸡的法氏囊功能和免疫状态。在通过法氏囊导管给予抗原后封闭该导管，被证明是一种严格通过法氏囊系统对鸡进行免疫的可靠且可重复的方法。在这种方法中，分离出的法氏囊中碳的形态和内吞作用正常。此外，手术鸡的抗布鲁氏菌滴度与未手术的经肛门免疫对照鸡相比无显著差异，而与对照相比，手术鸡对SRBC的反应显著降低（P小于0.001）。将法氏囊与GALT的其余部分分离表明，鸡法氏囊作为外周淋巴器官发挥作用；针对引入法氏囊腔的抗原形成特异性抗体。该手术方法还证实布鲁氏菌是一种依赖法氏囊的抗原，但SRBC需要GALT的协同作用才能产生良好的体液免疫反应。
