Matsuda H, Baba T, Bito Y
Immunology. 1975 Aug;29(2):307-18.
Surgically bursectomized and irradiated chickens were given bursal, splenic, bone marrow or thymic cells taken from syngeneic donors, together with killed Brucella abortus and Salmonella pullorum. Blood samples were taken from those chickens 7 days later, and the serum agglutinin titres were determined. The cells of any lymphoid organ taken from 28-day-old chickens were more effective in restoring antibody response than those from 18-day-old ones. The restorative capacities of the bursa and splenic cells were greater than those of the bone marrow and thymic cells. On the other hand, splenic, bone marrow or thymic cells taken from bursa-less chickens, and lymphoid cells taken from normal chickens but treated with anti-bursa serum in the persence of complement, were virtually incapable of restoring the immune response. Bursal, splenic or bone marrow cells taken from neonatally thymectomized chickens, and bursal or thymic cells treated with anti-thymus serum were effective, being comparable with the corresponding cells taken from normal chickens or treated with normal sera, in restoring the suppressed immune response in chickens devoid of the lymphoid system. These facts clearly indicate that the primary and central agent crucial for development of the humoral immune response against the two bacterial antigens tested is the bursa. It is strongly suggested from the results of adoptive immunization using intrabursally primed cells that the cells recognizing Brucella abortus exist within the bursa of 4-day-old chicken.
对通过手术切除法氏囊并进行辐照的鸡，给予取自同基因供体的法氏囊、脾脏、骨髓或胸腺细胞，同时注射灭活的流产布鲁氏菌和鸡白痢沙门氏菌。7天后采集这些鸡的血样，测定血清凝集素效价。取自28日龄鸡的任何淋巴器官的细胞，在恢复抗体反应方面比取自18日龄鸡的细胞更有效。法氏囊和脾脏细胞的恢复能力大于骨髓和胸腺细胞。另一方面，取自无囊鸡的脾脏、骨髓或胸腺细胞，以及取自正常鸡但在补体存在下用抗法氏囊血清处理的淋巴细胞，实际上无法恢复免疫反应。取自新生期胸腺切除鸡的法氏囊、脾脏或骨髓细胞，以及用抗胸腺血清处理的法氏囊或胸腺细胞，在恢复缺乏淋巴系统的鸡的受抑制免疫反应方面是有效的，与取自正常鸡或用正常血清处理的相应细胞相当。这些事实清楚地表明，对于针对所测试的两种细菌抗原的体液免疫反应的发育至关重要的主要和中心因子是法氏囊。从使用囊内致敏细胞进行过继免疫的结果强烈表明，识别流产布鲁氏菌的细胞存在于4日龄鸡的法氏囊内。
