College of Agriculture and Life Sciences, Kunming University, Kunming, Yunnan, PR China.
Lijiang Plant Protection Station, Lijiang, Yunnan, PR China.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005505.
A novel actinobacterium, designated KC 17012, was isolated from lead zinc tailings collected from Lanping, Yunnan, PR China. Comparative 16S rRNA gene sequencing showed that KC 17012 belonged to the genus and was most closely related to the type strains of (98.34%), (98.34%) and (98.27%). Phylogenetic tree analysis revealed strain KC 17012 formed a distinct clade. The genome size was 8.64 Mbp with a DNA G+C content of 70.8%. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain KC 17012 and those of JCM 4796 (25.3 and 81.5 %) and NBRC 109811 (30.1 and 85.7 %) were below the thresholds of 70 and 96% for prokaryotic conspecific assignation. The strain formed long straight aerial hyphae which generated regular short rod spores with spiny surfaces. Growth occurred at 10-45 °C, pH 6-8 and with 0-9 % NaCl (w/v). Strain KC 17012 contained ll-diaminopimelic acid and the major whole-cell hydrolysates included glucose, mannose and ribose. The menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids were iso-C, anteiso-C, iso-C and anteiso-C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified lipid and one unidentified phospholipid. On the basis of the results of a polyphasic taxonomic study, it is concluded that KC 17012 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain is KC 17012 (CGMCC 4.7704=JCM 35204).
一株新的放线菌，被命名为 KC17012，从中国云南兰坪采集的铅锌尾矿中分离得到。比较 16S rRNA 基因序列表明，KC17012 属于 和属，与 （98.34%）、 （98.34%）和 （98.27%）的模式菌株最为密切相关。系统发育树分析显示菌株 KC17012 形成了一个独特的分支。基因组大小为 8.64 Mbp，GC 含量为 70.8%。菌株 KC17012 与 JCM 4796（25.3 和 81.5%）和 NBRC 109811（30.1 和 85.7%）的基因组序列之间的数字 DNA-DNA 杂交和平均核苷酸同一性值均低于 70%和 96%，这是用于原核生物同物鉴定的阈值。该菌株形成长而直的气生菌丝，产生表面有刺的规则短杆孢子。生长温度为 10-45°C，pH 值为 6-8，盐度为 0-9%（w/v）。菌株 KC17012 含有 ll-二氨基庚二酸，主要的全细胞水解产物包括葡萄糖、甘露糖和核糖。其menaquinones 为 MK-9(H)、MK-9(H)和 MK-9(H)。主要细胞脂肪酸为 iso-C、anteiso-C、iso-C 和 anteiso-C。极性脂谱由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未鉴定的脂质和一种未鉴定的磷脂组成。基于多相分类学研究的结果，得出结论 KC17012 代表 属的一个新种，建议命名为 sp. nov.。模式菌株为 KC17012（CGMCC 4.7704=JCM 35204）。
