Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, 150030, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Antonie Van Leeuwenhoek. 2021 Jun;114(6):823-833. doi: 10.1007/s10482-021-01561-3. Epub 2021 Mar 27.
A novel endophytic actinomycete with antagonistic activity against various phytopathogenic fungi, designated strain p1417, was isolated from the root of cattail (Typha angustifolia L.) collected from Yunnan Province, Southwest China. A polyphasic taxonomic study was carried out to establish the taxonomic status of this strain. Strain p1417 was found to have morphological and chemotaxonomic characteristics typical of the genus Streptomyces. The diamino acid present in the cell wall was LL-diaminopimelic acid. Xylose and arabinose occurred in whole cell hydrolysates. The menaquinones were identified as MK-9(H), MK-9(H) and MK-9(H). The polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were found to be iso-C, anteiso-C iso-C and C. The genomic DNA G + C content of strain p1417 based on the genome sequence was 72.0 mol%. Based on 16 S rRNA gene, five housekeeping genes and whole genome sequences analysis, strain p1417 was most closely related to Streptomyces flavofungini JCM 4753 (99.4% 16 S rRNA gene sequence similarity), Streptomyces alboflavus JCM 4615 (98.8%) and Streptomyces aureoverticillatus JCM 4347 (98.2%). However, the average nucleotide identity values, the digital DNA-DNA hybridization values and the multilocus sequence analysis evolutionary distances between this strain and its closely related strains showed that it belonged to one distinct species. In addition, these results were also supported by differences in the phenotypic and chemotaxonomic characteristics between strain p1417 and three closely related type strains. Therefore, it is concluded that strain p1417 represents a novel species of the genus of Streptomyces, for which the name Streptomyces typhae sp. nov. is proposed. The type strain is p1417 (= CCTCC AA 2019091 = DSM 110636).
一株具有拮抗多种植物病原真菌活性的新型内生放线菌，被命名为 p1417 菌株，从中国西南云南省采集的香蒲（Typha angustifolia L.）根部分离得到。进行了多相分类学研究，以确定该菌株的分类地位。p1417 菌株具有典型的链霉菌属的形态和化学分类特征。细胞壁中的二氨基庚二酸为 LL-二氨基庚二酸。全细胞水解液中含有木糖和阿拉伯糖。鉴定出的menaquinones 为 MK-9(H)、MK-9(H)和 MK-9(H)。极性脂谱分析表明，含有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要脂肪酸为异-C、anteiso-C iso-C 和 C。根据基因组序列，p1417 菌株的基因组 DNA G+C 含量为 72.0 mol%。基于 16S rRNA 基因、五个管家基因和全基因组序列分析，p1417 菌株与链霉菌黄腐菌素 JCM 4753（99.4% 16S rRNA 基因序列相似性）、链霉菌白黄变种 JCM 4615（98.8%）和链霉菌金黄色变种 JCM 4347（98.2%）最为密切相关。然而，该菌株与近亲菌株之间的平均核苷酸同一性值、数字 DNA-DNA 杂交值和多位序列分析进化距离表明，它属于一个独特的种。此外，p1417 菌株与三个近亲型菌株之间表型和化学分类特征的差异也支持了这一结果。因此，推断 p1417 菌株代表链霉菌属的一个新种，命名为香蒲链霉菌（Streptomyces typhae sp. nov.）。模式菌株为 p1417（=CCTCC AA 2019091=DSM 110636）。
