Laboratory of Physiology/Pharmacology, Unit of Pathophysiology, Bioactive Substances and Safety, Faculty of Sciences, University of Lomé, BP 1515, Togo.
Laboratory of Physiology/Pharmacology, Unit of Pathophysiology, Bioactive Substances and Safety, Faculty of Sciences, University of Lomé, BP 1515, Togo.
Biomed Pharmacother. 2022 Oct;154:113578. doi: 10.1016/j.biopha.2022.113578. Epub 2022 Aug 23.
The aim of this study was to evaluate the antidiabetic properties of hydro alcoholic extract and supernatant fraction of the roots of Anogeissus leiocarpus, a plant used by traditional healers to treat Diabetes mellitus. Diabetes mellitus was induced by a single intraperitoneal administration of Streptozocin to Sprague Dawley rats under a fructose-enriched fat diet. Diabetic rats were treated with 500 mg/kg of total extract and 100 mg/kg of supernatant. The antidiabetic activity was assessed by measuring blood glucose level, lipid profile, insulin and biochemical parameters together with the antioxidant potential. The administration of total extract and supernatant exhibited significant decrease (p < 0.01) of the blood glucose level in the diabetic rats after 7 days of treatment compared to the diabetic rats. A significant reduction in the serum concentrations of cholesterol (19.7 %) and triglycerides (56.7 %) was observed in the treated diabetic rats. The levels of insulin did not differ across all the groups. However, compared to diabetic rats, HOMA-IR (Homeostasis Model Assessment for Insulin-resistance) and HOMA-β (Homeostasis Model Assessment for β cell function) showed a statistical decrease in insulin resistance and an increase in pancreatic β cell function in the treated diabetic rats. Moreover, total extract and supernatant significantly increased GSH level and decreased lipid peroxidation because of their antioxidant properties. In comparison, the supernatant fraction exerted stronger antidiabetic and antioxidant effects than the total extract. Hence, the roots of Anogeissus leiocarpus are a potent antidiabetic agent that can be developed as an alternative medicine for diabetes and its complications.
本研究旨在评估醉鱼草根的水醇提取物和上清部分的抗糖尿病特性，传统治疗师用该植物治疗糖尿病。糖尿病是通过在富含果糖的高脂肪饮食下向 Sprague Dawley 大鼠单次腹腔内给予链脲佐菌素诱导的。用 500mg/kg 总提取物和 100mg/kg 上清液治疗糖尿病大鼠。通过测量血糖水平、脂质谱、胰岛素和生化参数以及抗氧化能力来评估抗糖尿病活性。与糖尿病大鼠相比，总提取物和上清液给药后 7 天，糖尿病大鼠的血糖水平显著降低（p<0.01）。在治疗的糖尿病大鼠中，血清胆固醇（19.7%）和甘油三酯（56.7%）浓度显著降低。各组胰岛素水平无差异。然而，与糖尿病大鼠相比，HOMA-IR（胰岛素抵抗的稳态模型评估）和 HOMA-β（β细胞功能的稳态模型评估）显示胰岛素抵抗降低和胰腺β细胞功能增加。此外，由于其抗氧化特性，总提取物和上清液显著增加了 GSH 水平并降低了脂质过氧化。相比之下，上清部分比总提取物表现出更强的抗糖尿病和抗氧化作用。因此，醉鱼草根是一种有效的抗糖尿病药物，可以开发为治疗糖尿病及其并发症的替代药物。
