State Key Laboratry of Separation Membrane and Membrane Process, Tiangong University, Tianjin 300387, China; Tianjin Key Laboratory of Engineering Technologies for Cell Pharmaceutical, National Engineering Research Center of Cell Products, AmCellGene Co., Ltd., Tianjin 300457, China.
State Key Laboratry of Separation Membrane and Membrane Process, Tiangong University, Tianjin 300387, China.
J Photochem Photobiol B. 2022 Sep;234:112541. doi: 10.1016/j.jphotobiol.2022.112541. Epub 2022 Aug 11.
Acute lung injury (ALI) impaired the function of blood oxygen exchange function, resulting in tissue hypoxia and patient death. Recently, human umbilical cord mesenchymal stem cells (hUCMSCs) are thought to mitigate the effects of ALI, which boosts researchers' interest in employing stem cell-based therapies to manage ALI. However, as a novel therapy, hUCMSCs still face various limitations such as migrating weakly and insufficient proliferation in vivo. Photobiomodulation (PBM) effciently promotes cell proliferation, migration and homing, which presents a promising strategy for overcoming above limitations. In this study, PBM was emerged to intervene hUCMSCs through detecting cell proliferation, oxidative stress-related factors and inflammatory factors. These results assuredly confirmed that PBM enhanced the antioxidant capacity of cells and improved cell survival in vitro experiments. In vivo, PBM-intervened hUCMSCs intuitively reduce thickness of alveolar septum, excessive secretion of inflammatory factors, relieves bleeding, edema and fibrosis. As a physical intervention, PBM further strengthens the therapeutic effect of hUCMSCs and depicted a hopeful therapy in ALI treatment.
急性肺损伤（ALI）损害了血液氧交换功能，导致组织缺氧和患者死亡。最近，人们认为人脐带间充质干细胞（hUCMSCs）可以减轻 ALI 的影响，这激发了研究人员对基于干细胞的疗法来治疗 ALI 的兴趣。然而，作为一种新的疗法，hUCMSCs 仍然面临着各种限制，如体内迁移能力弱和增殖不足。光生物调节（PBM）有效地促进细胞增殖、迁移和归巢，为克服上述限制提供了一种很有前途的策略。在这项研究中，通过检测细胞增殖、氧化应激相关因子和炎症因子，PBM 被用来干预 hUCMSCs。这些结果肯定证实了 PBM 增强了细胞的抗氧化能力，并提高了细胞在体外实验中的存活率。在体内，PBM 干预的 hUCMSCs 明显减轻了肺泡隔的厚度、过度分泌炎症因子、减轻了出血、水肿和纤维化。作为一种物理干预，PBM 进一步增强了 hUCMSCs 的治疗效果，为 ALI 的治疗描绘了一种有希望的治疗方法。
