在分析特应性儿童食物过敏方面，ALEX与ISAC多重检测阵列的比较

ALEX versus ISAC multiplex array in analyzing food allergy in atopic children.

作者信息

Sonneveld Laura J H, Emons Joyce A M, Arends Nicolette J T, Landzaat Lonneke J, Veenbergen Sharon, Schreurs Marco W J

机构信息

Department of Pediatrics, Division of Respiratory Medicine and Allergology, Sophia Children Hospital, Erasmus MC University Medical Centre, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.

Department of Immunology, Laboratory Medical Immunology, Erasmus MC University Medical Centre, Rotterdam, The Netherlands.

出版信息

Clin Mol Allergy. 2022 Aug 27;20(1):10. doi: 10.1186/s12948-022-00177-w.

DOI:10.1186/s12948-022-00177-w

PMID:36030246

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9419344/

Abstract

ALEX multiplex array is a relatively new multiplex allergy test which analyses more than 120 allergen extracts and 170 molecular components. ISAC is the most used and studied multiplex array to date, offering 112 molecular components. In ten atopic children with multiple food allergies good agreement was observed between ALEX and ISAC sIgE results for nearly all shared food components. Presence of larger number of allergens in ALEX could help clinicians to improve personalized dietary advice. However more positive sensitizations with unknown clinical relevance were found by ALEX, potentially increasing clinical complexity. Pediatric allergists should be aware of this, especially in young atopic children with (severe) eczema who have not introduced all sorts of food yet.

摘要

ALEX多重检测阵列是一种相对较新的多重过敏检测方法，可分析120多种过敏原提取物和170种分子成分。ISAC是迄今为止使用最广泛且研究最多的多重检测阵列，提供112种分子成分。在10名患有多种食物过敏的特应性儿童中，对于几乎所有共同的食物成分，ALEX和ISAC的特异性免疫球蛋白E（sIgE）结果之间观察到良好的一致性。ALEX中存在更多数量的过敏原可能有助于临床医生改进个性化饮食建议。然而，ALEX发现了更多具有未知临床相关性的阳性致敏反应，这可能会增加临床复杂性。儿科过敏症专科医生应意识到这一点，尤其是在尚未引入各种食物的患有（重度）湿疹的年轻特应性儿童中。