Centre for Synthetic Biology (CSB), Ghent University, Coupure links 653, 9000 Ghent, Belgium.
Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
Biotechnol Adv. 2022 Nov;60:108028. doi: 10.1016/j.biotechadv.2022.108028. Epub 2022 Aug 27.
Synthetic biology can play a major role in the development of sustainable industrial biotechnology processes. However, the development of economically viable production processes is currently hampered by the limited availability of host organisms that can be engineered for a specific production process. To date, standard hosts such as Escherichia coli and Saccharomyces cerevisiae are often used as starting points for process development since parts and tools allowing their engineering are readily available. However, their suboptimal metabolic background or impaired performance at industrial scale for a desired production process, can result in increased costs associated with process development and/or disappointing production titres. Building a universal and portable gene expression system allowing genetic engineering of hosts across the bacterial domain would unlock the bacterial domain for industrial biotechnology applications in a highly standardized manner and, doing so, render industrial biotechnology processes more competitive compared to the current polluting chemical processes. This review gives an overview of a selection of bacterial hosts highly interesting for industrial biotechnology based on both their metabolic and process optimization properties. Moreover, the requirements and progress made so far to enable universal, standardized, and portable gene expression across the bacterial domain is discussed.
合成生物学可以在可持续工业生物技术工艺的发展中发挥重要作用。然而，经济可行的生产工艺的发展目前受到可用于特定生产工艺的工程宿主生物的有限可用性的阻碍。迄今为止，标准宿主，如大肠杆菌和酿酒酵母，通常被用作工艺开发的起点，因为允许对其进行工程改造的部件和工具易于获得。然而，它们在所需生产工艺方面的代谢背景不佳或在工业规模下的性能不佳，可能会导致与工艺开发相关的成本增加和/或生产产量令人失望。构建允许跨细菌域对宿主进行基因工程的通用且可移植的基因表达系统，将以高度标准化的方式为工业生物技术应用解锁细菌域，并使工业生物技术工艺相对于当前污染严重的化学工艺更具竞争力。本综述概述了一些基于代谢和工艺优化特性对工业生物技术极具吸引力的细菌宿主。此外，还讨论了实现跨细菌域通用、标准化和可移植基因表达的要求和迄今取得的进展。
