He Jian, Shang Xiaofei, Dai Lixia, Yang Xiaorong, Li Bing, Wei Yanming, Zhang Jiyu, Pan Hu
College of Veterinary Medicines, Gansu Agricultural University, Lanzhou, China.
Key Laboratory of New Animal Drug Project, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Front Vet Sci. 2022 Aug 10;9:882060. doi: 10.3389/fvets.2022.882060. eCollection 2022.
As the ornamental plants and traditional medicines, , and are widely distributed in western China. In this paper, the essential oils from these four species were extracted by supercritical extraction and the components were analyzed using headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS), the antibacterial, acaricidal and anti-inflammatory activities were investigated. Results showed that (RTEO) contained the highest yield of 0.99% with 246 compounds, followed by (RCEO, 0.81%) with 290 chemicals, (RAEO, 0.57%) with 302 compounds and (RPEO, 0.30%) with 294 components. They also exhibited the safety at given doses and have the anti-inflammatory and tests inhibiting the cytokines productions, the acaricidal and antibacterial activities also were found. 4-Hydroxy-3-methylacetophenone from RPEO, α-pinene and β-pinene from RAEO, β-farnesene and germacrone from RTEO, and benzylacetone from RCEO, as main and active components, inhibited the NO content in RAW 264.7 cells induced by LPS. These results indicated that four essential oils have certain medicinal value and laid the foundation for the development of these species as raw materials for the pharmaceutical and perfume industries.
作为观赏植物和传统药物，[具体植物名称1]、[具体植物名称2]、[具体植物名称3]和[具体植物名称4]在中国西部广泛分布。本文采用超临界萃取法提取这四种植物的挥发油，并采用顶空固相微萃取结合气相色谱-质谱联用仪（HS-SPME-GC-MS）对其成分进行分析，同时研究了它们的抗菌、杀螨和抗炎活性。结果表明，[具体植物名称1]挥发油（RTEO）得率最高，为0.99%，含有246种化合物；其次是[具体植物名称2]挥发油（RCEO，0.81%），含有290种化学成分；[具体植物名称3]挥发油（RAEO，0.57%）含有302种化合物；[具体植物名称4]挥发油（RPEO，0.30%）含有294种成分。它们在给定剂量下也表现出安全性，具有抗炎作用，在[具体抗炎测试]中抑制细胞因子的产生，同时还具有杀螨和抗菌活性。来自RPEO的4-羟基-3-甲基苯乙酮、来自RAEO的α-蒎烯和β-蒎烯、来自RTEO的β-法尼烯和吉马酮以及来自RCEO的苄基丙酮作为主要活性成分，抑制了脂多糖诱导的RAW 264.7细胞中NO的含量。这些结果表明，四种挥发油具有一定的药用价值，为将这些植物开发为制药和香料工业的原料奠定了基础。
