Maseno University, Department of Biomedical Sciences & Technology; Masinde Muliro University of Science and Technology, Department of Biological sciences.
Masinde Muliro University ofScience and Technology, Department of Biological sciences.
Afr Health Sci. 2022 Mar;22(1):589-597. doi: 10.4314/ahs.v22i1.68.
Insecticide treated bed nets and Indoor residual spraying remains the principal interventional malaria control strategies. To achieve malaria disease eradication, vector control programmes that monitor insecticide resistance profiles are necessary.
The study evaluated pirimiphos-methyl susceptibility of sensu lato in Kakamega County, western Kenya.
Adult sensu lato mosquitoes were assayed using World Health Organization tube bioassay against 0.25% pirimiphos-methyl. Susceptible and non-susceptible populations were characterized to species-level using Polymerase Chain Reaction. Susceptible and resistant mosquitoes were further subjected to G119S Acetylcholisterase (ace 1R) mutation detection.
was the predominant species in all study population Mumias east (62%), Malava (68%), Ikolomani (77%) and Lurambi (82%). Results showed phenotypic susceptibility to pirimiphos-methyl. Mortality was low in Mumias east (80.6%) and high in Lurambi (89.0%). G119S mutations ranged from 3.0% to 8.9% in whereas G119S mutations were relatively low ranging from 0.0% to 3.1% in s.s populations. Study populations tested were consistent with Hardy-Weinberg equilibrium (P>0.05).
We observed pirimiphos-methyl resistance in Anopheles arabiensis and s.s. study populations. Results showed G119S mutation in resistance population. Resistance monitoring and management are urgently required.
经杀虫剂处理的蚊帐和室内滞留喷洒仍然是主要的干预性疟疾控制策略。为了实现消除疟疾，有必要对监测杀虫剂耐药性特征的病媒控制方案进行监测。
本研究评估了肯尼亚西部卡卡梅加县广义按蚊对吡虫啉的敏感性。
使用世界卫生组织管生物测定法，对 0.25%吡虫啉处理的成年广义按蚊进行了检测。使用聚合酶链反应将敏感和非敏感种群特征鉴定到种水平。对敏感和抗性蚊子进一步进行 G119S 乙酰胆碱酯酶（ace 1R）突变检测。
在所研究的所有种群中，均为优势种，Mumias east（62%）、Malava（68%）、Ikolomani（77%）和 Lurambi（82%）。结果表明对吡虫啉表现出表型敏感性。Mumias east 的死亡率较低（80.6%），而 Lurambi 的死亡率较高（89.0%）。G119S 突变在 中范围为 3.0%至 8.9%，而在 s.s 种群中，G119S 突变相对较低，范围为 0.0%至 3.1%。所测试的研究种群与 Hardy-Weinberg 平衡一致（P>0.05）。
我们观察到在埃及伊蚊和 s.s 研究种群中存在吡虫啉抗药性。结果显示在抗性种群中存在 G119S 突变。迫切需要进行抗药性监测和管理。
