使用海绵垫法并注入 fertile 包虫囊肿，评估高渗盐水、硝酸银、乙醇的原头蚴杀灭效果。 （注：原文中“fertile”在医学语境下可能不太准确，推测这里想表达的是“有活性的”等类似意思，比如“active” ，但按照要求未作修改直接翻译）
Evaluation of the Protoscolicidal Effectiveness of Hypertonic Saline, Silver Nitrate, Ethanol, Using Sponge Pad Method and Injecting into Fertile Hydatid Cysts.
作者信息
Fakharzadeh Jahromi Kereshmeh, Rafiei Abdollah, Rahdar Mahmoud, Bahreini Amin
机构信息
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Parasitology, School of Medicine, Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Iran J Parasitol. 2022 Apr-Jun;17(2):223-230. doi: 10.18502/ijpa.v17i2.9540.
BACKGROUND
We aimed to evaluate currently protoscolices agents by using two methods including injection and impregnated sponges with protoscolices agents.
METHODS
Hypertonic saline 20%, silver nitrate 0.5% and Ethanol 96% were injected into sheep hydatid cysts. Then, after different exposure times (5, 10, and 15 min), the protoscolices were aspirated from the cyst and viability of the protoscolices were evaluated microscopically using vital staining. In the second part, the live protoscolices were sprayed on the soaked sponge with the mentioned protoscolicidal agents. After different exposure times live protoscolices were counted microscopically. One-way ANOVA and Tukey tests were used for showing difference significant between studied groups.
RESULTS
Cyst injection method showed that ethanol, Nacl and AgNo3 have 80%, 60% and 6% protoscolocidal efficacy, respectively (<0.05). In sponge method the most and fastest protoscolocidal efficacy were observed in all used protoscolicidal agents.
CONCLUSION
Ethanol and NaCl and AgNo3 with sponge method is more effective than the injection of protoscolices agents in the cyst.
背景
我们旨在通过两种方法评估目前的原头蚴杀灭剂，这两种方法包括注射以及用原头蚴杀灭剂浸渍海绵。
方法
将20%的高渗盐水、0.5%的硝酸银和96%的乙醇注入绵羊包虫囊肿。然后，在不同的暴露时间（5、10和15分钟）后，从囊肿中吸出原头蚴，并使用活体染色在显微镜下评估原头蚴的活力。在第二部分中，将活的原头蚴喷洒在浸泡有上述原头蚴杀灭剂的海绵上。在不同的暴露时间后，在显微镜下对活的原头蚴进行计数。使用单向方差分析和Tukey检验来显示研究组之间的显著差异。
结果
囊肿注射法显示，乙醇、氯化钠和硝酸银的原头蚴杀灭效果分别为80%、60%和6%（P<0.05）。在海绵法中，在所有使用的原头蚴杀灭剂中均观察到最高且最快的原头蚴杀灭效果。
结论
乙醇、氯化钠和硝酸银与海绵法联合使用比在囊肿中注射原头蚴杀灭剂更有效。
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