活化的血小板膜纳米囊泡募集中性粒细胞以发挥抗肿瘤功效。
Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency.
作者信息
Shang Yinghui, Sun Juntao, Wu Xin, Wang Qinghai
机构信息
Department of Hematology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Gastroenterology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
出版信息
Front Chem. 2022 Aug 11;10:955995. doi: 10.3389/fchem.2022.955995. eCollection 2022.
Platelets play a crucial role in the recruitment of neutrophils, mediated by P-selectin, CCL5, and ICAM-2. In this study, we prepared platelet membrane nanovesicles from activated platelets. Whether activated platelet membrane nanovesicles can recruit neutrophils has not been reported, nor has their role in antitumor immunity. The results of SDS-PAGE showed that the platelet membrane nanovesicles retained almost all the proteins of platelets. Western blotting showed that both the activated platelets and the platelet membrane nanovesicles expressed P-selectin, ICAM-2, and CCL5. results of a mouse model of breast cancer-transplanted tumor showed that tumor volume reduced significantly, Ki-67-positive tumor cells decreased, and TUNEL-positive tumor cells increased in tumors after treatment with activated platelet membrane nanovesicles (aPNs). After treatment with aPNs, not only the number of neutrophils, CD8, CD4 T cells, and B cells increased, but also IL-12, TNF-α, and IFN-γ levels elevated significantly in tumor tissues.
血小板在由P-选择素、CCL5和ICAM-2介导的中性粒细胞募集中发挥关键作用。在本研究中，我们从活化血小板制备了血小板膜纳米囊泡。活化血小板膜纳米囊泡是否能募集中性粒细胞尚未见报道，其在抗肿瘤免疫中的作用也未见报道。SDS-PAGE结果显示，血小板膜纳米囊泡保留了血小板几乎所有的蛋白质。蛋白质免疫印迹法显示，活化血小板和血小板膜纳米囊泡均表达P-选择素、ICAM-2和CCL5。乳腺癌移植瘤小鼠模型的结果显示，用活化血小板膜纳米囊泡（aPNs）治疗后，肿瘤体积显著减小，Ki-67阳性肿瘤细胞减少，TUNEL阳性肿瘤细胞增加。用aPNs治疗后，不仅肿瘤组织中中性粒细胞、CD8、CD4 T细胞和B细胞的数量增加，而且IL-12、TNF-α和IFN-γ水平也显著升高。
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