用于测量小鼠脑中转录因子活性的病毒载体方法的协议。
Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain.
机构信息
Lab of Brain Development, Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aobaku, Sendai, Miyagi 980-8577, Japan.
Lab of Brain Development, Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aobaku, Sendai, Miyagi 980-8577, Japan; Division for the Establishment of Frontier Science, Tohoku University, Katahira 2-1-1, Aobaku, Sendai, Miyagi 980-8577, Japan.
出版信息
STAR Protoc. 2022 Aug 18;3(3):101633. doi: 10.1016/j.xpro.2022.101633. eCollection 2022 Sep 16.
Here, we provide a step-by-step protocol to measure the activities of multiple transcription factors (TFs) in the same mouse brain. This protocol includes a procedure to construct a virus-based TF activity reporter, transfection, and PCR-based measurement of TF activity to obtain the transcription factor activity profile (TFAP). Our protocol facilitates a systematic analysis of TF activity of the brain and will aid trans-omics understanding of the molecular mechanism underlying the brain functions. For complete details on the use and execution of this protocol, please refer to Abe and Abe, (2022).
在这里，我们提供了一个逐步的方案来测量同一老鼠大脑中的多个转录因子 (TFs) 的活性。本方案包括构建基于病毒的 TF 活性报告基因、转染和基于 PCR 的 TF 活性测量的程序，以获得转录因子活性图谱 (TFAP)。我们的方案促进了大脑中 TF 活性的系统分析，并将有助于对大脑功能背后的分子机制的跨组学理解。有关该方案使用和执行的完整详细信息，请参阅 Abe 和 Abe（2022 年）。
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