Department of Aquaculture, College of Animal Science & Technology, Jiangxi Agricultural University, Nanchang, P.R. China.
Jiangxi Fisheries Research Institute, Nanchang, P.R. China.
Vet Med Sci. 2022 Nov;8(6):2404-2410. doi: 10.1002/vms3.918. Epub 2022 Aug 29.
Enrofloxacin (ENR) is a kind of quinolone antibiotic that is most widely used antimicrobials in veterinary practice, and possesses both a broad spectrum antimicrobial activity against a range of bacteria and adverse effects towards plants and animals.
This study was conducted to explore the permeability of blood-brain barrier (BBB) to ENR and brain injury based on crucian carp orally administrated with high dose of ENR.
Juvenile Pengze crucian carp were treated with half lethal dose (LD ) or safe dose (SD ) of ENR. BBB permeability was determined by evaluating ENR contents detected by HPLC and evens blue contents estimated by confocal laser scanning microscope. Brain damage was evaluated by measuring protein and mRNA contents of related molecules with western blotting and qPCR.
Data indicated that ENR destroyed BBB structure of crucian carp and enhanced permeability of the biological barrier, resulting in more ENR crossed BBB and induced brain damage of crucian carp.
This data indicated that ENR can induce brain damage of crucian carp through destroying BBB structure and enhancing permeability.
恩诺沙星（ENR）是一种喹诺酮类抗生素，在兽医实践中被广泛用作抗菌药物，对多种细菌具有广谱抗菌活性，同时对植物和动物也有不良影响。
本研究旨在通过鲫鱼口服高剂量恩诺沙星，探讨其对血脑屏障（BBB）的通透性和脑损伤的影响。
采用半数致死量（LD）或安全剂量（SD）的恩诺沙星处理幼龄彭泽鲫鱼。通过高效液相色谱法（HPLC）检测恩诺沙星含量和共聚焦激光扫描显微镜检测伊文思蓝含量来评估 BBB 的通透性。采用 Western blot 和 qPCR 测定相关分子的蛋白和 mRNA 含量来评估脑损伤。
数据表明，恩诺沙星破坏了鲫鱼的 BBB 结构，增加了生物屏障的通透性，导致更多的恩诺沙星穿过 BBB，并引起鲫鱼的脑损伤。
本研究数据表明，恩诺沙星通过破坏 BBB 结构和增加通透性，可引起鲫鱼的脑损伤。
