Ministry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
Anal Bioanal Chem. 2022 Nov;414(26):7731-7740. doi: 10.1007/s00216-022-04304-z. Epub 2022 Aug 30.
Realizing the rapid and on-site detection of biothiols in complex biological and food samples using simple assays and devices remains a major challenge. In this study, biothiols containing sulfhydryl groups were found to be able to inhibit the photo-triggered oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). Based on the discovery, using the commercially available and low-cost TMB as the chromogenic substrate, an enzyme-free colorimetric approach was developed for the rapid determination of biothiols. The method does not involve the introduction of any natural enzymes, nanoenzymes, and external oxidants. The mechanisms of the photoinduced oxidation of TMB and the detection of biothiols were proposed. Furthermore, a smartphone-based portable device integrated with test strips was constructed by the 3D printing technique. This device can simultaneously meet the requirements of the photocatalytic oxidation reaction of TMB and the detection of biothiols. The entire process only takes less than 5 min. The successful detection of cysteine in urine and milk samples demonstrates the great potential of the device in the on-site assays.
实现使用简单的检测手段和设备，快速、现场检测复杂生物和食品样品中的生物硫醇仍然是一个重大挑战。在这项研究中，发现含有巯基的生物硫醇能够抑制 3,3',5,5'-四甲基联苯胺（TMB）的光触发氧化。基于这一发现，利用市售且廉价的 TMB 作为显色底物，开发了一种无需引入任何天然酶、纳米酶和外部氧化剂的酶免比色法，用于快速测定生物硫醇。提出了 TMB 光诱导氧化和生物硫醇检测的机制。此外，还通过 3D 打印技术构建了一种基于智能手机的集成测试条的便携式设备。该设备可以同时满足 TMB 的光催化氧化反应和生物硫醇检测的要求。整个过程只需不到 5 分钟。该设备成功检测了尿液和牛奶样本中的半胱氨酸，证明了该设备在现场检测中的巨大潜力。
