S. Maria Hospital Terni, Terni, Italy.
IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy.
PLoS One. 2022 Aug 30;17(8):e0273679. doi: 10.1371/journal.pone.0273679. eCollection 2022.
In central nervous system neurodegenerative disorders, stem cell-based therapies should be considered as a promising therapeutic approach. The safe use of human Neural Stem Cells (hNSCs) for the treatment of several neurological diseases is currently under evaluation of phase I/II clinical trials. Clinical application of hNSCs require the development of GMP standardized protocols capable of generating high quantities of reproducible and well characterized stem cells bearing stable functional and genetic properties.
The aim of this study was to evaluate possible instabilities or modifications of the microsatellite loci in different culture passages because high culture passages represent an in vitro replicative stress leading to senescence. Experimental method: The hNSCs were characterized at different culture time points, from passage 2 to passage 25, by genetic typing at ten microsatellite loci.
We showed that genetic stability at microsatellite loci is maintained by the cells even at high passages adding a further demonstration of the safety of our hNSCs GMP culture method.
在中枢神经系统神经退行性疾病中，基于干细胞的疗法应被视为一种有前途的治疗方法。目前正在评估 I/II 期临床试验，以安全地使用人神经干细胞（hNSCs）治疗多种神经疾病。hNSCs 的临床应用需要开发符合 GMP 标准的协议，这些协议能够生成大量具有稳定功能和遗传特性的可重复和特征良好的干细胞。
本研究旨在评估不同培养传代中微卫星位点的可能不稳定性或修饰，因为高传代会导致体外复制应激导致衰老。实验方法：通过对十个微卫星位点进行遗传分型，在从第 2 代到第 25 代的不同培养时间点对 hNSCs 进行特征描述。
即使在高传代时，细胞也能保持微卫星位点的遗传稳定性，进一步证明了我们的 hNSCsGMP 培养方法的安全性。
