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[作为受体配体的放射性性激素的纯化]

[Purification of radioactive sex hormones as receptor ligands].

作者信息

Degtiar' V G, Kushlinskiĭ N E, Khotchenkova N V

出版信息

Vopr Med Khim. 1987 Mar-Apr;33(2):71-3.

PMID:3604146
Abstract

A system (mobile phase) containing benzene:acetone:absolute ethanol 9:1:0.5, respectively, is described for thin-layer chromatography on Silufol plates of ligand receptors of sex steroid hormones 3H-5 alpha-androstan-3 beta, 17 beta-diol, 3H-estradiol-17 beta, 3H-5 alpha-dihydrotestosterone, 3H-methyltrienolone (R 1881), 3H-OPG 2058 and 3H-progesterone. A purity of commercially available 3H-steroids was decreased in storage, therefore they should be purified before use. The system described enabled to produce the 3H-steroids studied of high purity.

摘要

一种分别含有苯

丙酮:无水乙醇比例为9:1:0.5的系统(流动相),用于在Silufol板上对性类固醇激素配体受体进行薄层色谱分析,这些受体包括3H-5α-雄甾烷-3β,17β-二醇、3H-雌二醇-17β、3H-5α-双氢睾酮、3H-甲基三烯olone(R 1881)、3H-OPG 2058和3H-孕酮。市售的3H-类固醇在储存过程中纯度会降低,因此在使用前应进行纯化。所描述的系统能够制备出高纯度的所研究的3H-类固醇。

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