Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Howard Hughes Medical Institute, Boston, MA, USA.
BMC Genomics. 2022 Aug 30;23(1):623. doi: 10.1186/s12864-022-08845-6.
The pathophysiological effects of a number of metabolic and age-related disorders can be prevented to some extent by exercise and increased physical activity. However, the molecular mechanisms that contribute to the beneficial effects of muscle activity remain poorly explored. Availability of a fast, inexpensive, and genetically tractable model system for muscle activity and exercise will allow the rapid identification and characterization of molecular mechanisms that mediate the beneficial effects of exercise. Here, we report the development and characterization of an optogenetically-inducible muscle contraction (OMC) model in Drosophila larvae that we used to study acute exercise-like physiological responses. To characterize muscle-specific transcriptional responses to acute exercise, we performed bulk mRNA-sequencing, revealing striking similarities between acute exercise-induced genes in flies and those previously identified in humans. Our larval muscle contraction model opens a path for rapid identification and characterization of exercise-induced factors.
许多代谢和与年龄相关的疾病的病理生理效应在一定程度上可以通过运动和增加身体活动来预防。然而，肌肉活动有益效果的分子机制仍未得到充分探索。拥有一种快速、廉价和遗传上易于处理的肌肉活动和运动模型系统，将使快速识别和鉴定介导运动有益效果的分子机制成为可能。在这里，我们报告了在果蝇幼虫中开发和鉴定的一种光遗传学诱导的肌肉收缩（OMC）模型，我们使用该模型来研究类似急性运动的生理反应。为了鉴定肌肉特异性转录对急性运动的反应，我们进行了批量 mRNA 测序，结果表明果蝇中急性运动诱导基因与之前在人类中鉴定的基因之间存在惊人的相似性。我们的幼虫肌肉收缩模型为快速鉴定和鉴定运动诱导因子开辟了道路。
