Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 6340034, Russian Federation.
JST-ERATO Yamauchi Materials Space-Tectonics Project and International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
ACS Appl Mater Interfaces. 2022 Sep 14;14(36):41629-41639. doi: 10.1021/acsami.2c12804. Epub 2022 Aug 31.
For surface-enhanced Raman spectroscopy (SERS) sensing of small molecules in the presence of living cells, biofouling and blocking of plasmonic centers are key challenges. Here, we have developed a mesoporous Au (AuM) film coated with a Ag nanoparticles (NPs) as a plasmonic sensor (AuM@Ag) to analyze aromatic thiols, which is an example of a small molecule, in the presence of a living cell strain (e.g., MDA-MB-231) as a model living system. The resulting AuM@Ag provides 0.1 nM sensitivity and high reproducibility for thiols sensing. Simultaneously, the AuM@Ag film filters large biomolecules, preventing Raman signals from overlapping produced by large biomolecules. After analysis, the AuM@Ag film undergoes recycling by the full dissolution of the Ag-thiol layer and removal of thiols from AuM. Furthermore, fresh AgNPs are formed for further SERS analysis, which circumvents the Ag oxidation issue. The ease of the AgNPs deposition allows up to 12 cycles of on-demand recycling and sensing even after utilization as a sensor in multicomponent media without enhancement and sensitivity loss. The reported mesoporous film with surface filtering ability and prominent recycling procedure promises to offer a new strategy for the detection of various small molecules in the presence of living cells.
对于在活细胞存在下的小分子的表面增强拉曼光谱（SERS）传感，生物污垢和等离子体中心的阻断是关键挑战。在这里，我们开发了一种介孔 Au（AuM）膜，其表面涂覆有 Ag 纳米粒子（NPs）作为等离子体传感器（AuM@Ag），以分析小分子芳香硫醇，这是小分子的一个例子，以 MDA-MB-231 等活细胞株作为模型活系统。所得的 AuM@Ag 为硫醇传感提供了 0.1 nM 的灵敏度和高重现性。同时，AuM@Ag 薄膜过滤大分子生物分子，防止由大分子生物分子产生的 Raman 信号重叠。分析后，AuM@Ag 薄膜通过 Ag-硫醇层的完全溶解和 AuM 中硫醇的去除来进行回收。此外，形成新鲜的 AgNPs 以进行进一步的 SERS 分析，从而避免了 Ag 氧化问题。AgNPs 的沉积容易进行，即使在多组分介质中用作传感器后，也可以进行多达 12 次按需回收和传感循环，而不会降低增强和灵敏度。具有表面过滤能力和突出的回收程序的报道介孔薄膜有望为在活细胞存在下检测各种小分子提供一种新策略。
