Department of Systems Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.
Department of Experimental Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.
Acta Diabetol. 2022 Dec;59(12):1589-1596. doi: 10.1007/s00592-022-01956-2. Epub 2022 Aug 31.
Disturbances in circadian rhythms may promote cardiometabolic disorders in rotating night shift workers (r-NSWs). We hypothesized that timed light therapy might reverse disrupted circadian rhythms and glucose intolerance observed among r-NSWs).
R-NSWs were randomly assigned to a protocol that included 12 weeks on followed by 12 weeks off light therapy (n = 13; 6 men; mean age, 39.5 ± 7.3 years) or a no-treatment control group (n = 9; 3 men; mean age 41.7 ± 6.3 years). Experimental and control participants underwent identical metabolic evaluations that included anthropometric, metabolic (including oral glucose tolerance tests), lipid, and inflammation-associated parameters together with an assessment of sleep quality and expression of circadian transcription factors REV-ERBα and BMAL1 in peripheral blood mononuclear cells (PBMCs) at baseline, 12 weeks, and 24 weeks of the protocol.
Twelve weeks of warm white-light exposure (10,000 lx at 35 cm for 30 min per day) had no impact on sleep, metabolic, or inflammation-associated parameters among r-NSWs in the experimental group. However, our findings revealed significant decreases in REV-ERBα gene expression (p = 0.048) and increases in the REV-ERBα/BMAL1 ratio (p = 0.040) compared to baseline in PBMCs isolated from this cohort. Diminished expression of REV-ERBα persisted, although the REV-ERBα/BMAL1 ratio returned to baseline levels after the subsequent 12-day wash-out period.
Our results revealed that intermittent light therapy had no impact on inflammatory parameters or glucose tolerance in a defined cohort of r-NSWs. However, significant changes in the expression of circadian clock genes were detected in PBMCs of these subjects undergoing light therapy.
昼夜节律紊乱可能会促进轮班夜间工作者（r-NSW）的心脏代谢紊乱。我们假设定时光疗可能会逆转 r-NSW 中观察到的昼夜节律紊乱和葡萄糖不耐受。
r-NSW 被随机分配到一个方案中，该方案包括 12 周的光疗期和 12 周的光疗休息期（n=13；6 名男性；平均年龄 39.5±7.3 岁）或无治疗对照组（n=9；3 名男性；平均年龄 41.7±6.3 岁）。实验组和对照组接受了相同的代谢评估，包括人体测量学、代谢（包括口服葡萄糖耐量试验）、脂质和炎症相关参数，以及睡眠质量评估和外周血单核细胞（PBMCs）中昼夜转录因子 REV-ERBα 和 BMAL1 的表达，在方案的基线、12 周和 24 周进行。
12 周的暖色白光暴露（每天 35 厘米处 10,000 lx 照射 30 分钟）对实验组 r-NSW 的睡眠、代谢或炎症相关参数没有影响。然而，我们的发现表明，与基线相比，从该队列中分离的 PBMCs 中 REV-ERBα 基因表达显著降低（p=0.048），REV-ERBα/BMAL1 比值升高（p=0.040）。尽管在随后的 12 天洗脱期后，REV-ERBα 的表达恢复到基线水平，但 REV-ERBα 的表达仍然减弱。
我们的结果表明，间歇性光疗对特定 r-NSW 队列的炎症参数或葡萄糖耐量没有影响。然而，在接受光疗的这些受试者的 PBMCs 中，检测到昼夜节律钟基因表达的显著变化。
