Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering. University of Jinan, Jinan 250022, Shandong, China.
Shandong Academy of Environmental Science Co., Ltd., Jinan 250022, Shandong, China.
Anal Chem. 2022 Sep 13;94(36):12531-12537. doi: 10.1021/acs.analchem.2c02780. Epub 2022 Aug 31.
In this study, a portable electrochemiluminescence sensor chip was designed for trenbolone (TBE) trace detection in environmental water. First, a stable ECL signal was obtained with low-toxicity 3,4,9,10-perylenetetracarboxylic acid (PTCA) as a luminophore and persulfate (SO) as a coreactant. Second, hollow-structured CuMoS was introduced as a coreaction accelerator to catalyze SO reduction. The reversible conversion of the mixed-valence transition metal ions in CuMoS (Cu/Cu and Mo/Mo) greatly promoted the generation of the sulfate radical (SO). Meanwhile, the special porous structure of CuMoS possessed a large specific surface area, thus enhancing its catalytic performance. Based on these enhancement mechanisms, a strong ECL signal was acquired, which improved the detection sensitivity of the constructed sensor. Importantly, a microfluidic chip was introduced for sensing detection, thereby improving the practicality of the sensor. The developed sensor chip was miniature and portable, exhibiting high sensitivity for TBE detection with a wide linear range (10 fg/mL-100 ng/mL) and lower detection limit (3.32 fg/mL). This was of great significance for timely and rapid analysis of steroid pollutants in natural water.
在这项研究中，设计了一种用于痕量检测环境水中 trenbolone（TBE）的便携式电致化学发光传感器芯片。首先，以低毒性的 3,4,9,10-苝四羧酸（PTCA）作为发光体，过硫酸盐（SO）作为共反应物，获得了稳定的 ECL 信号。其次，引入中空结构的 CuMoS 作为共反应加速剂，以催化 SO 的还原。CuMoS 中混合价态过渡金属离子（Cu/Cu 和 Mo/Mo）的可逆转换极大地促进了硫酸根自由基（SO）的生成。同时，CuMoS 的特殊多孔结构具有较大的比表面积，从而增强了其催化性能。基于这些增强机制，获得了强的 ECL 信号，提高了所构建传感器的检测灵敏度。重要的是，引入了微流控芯片进行传感检测，从而提高了传感器的实用性。所开发的传感器芯片小巧便携，对 TBE 的检测具有较高的灵敏度，线性范围较宽（10 fg/mL-100 ng/mL），检测限较低（3.32 fg/mL）。这对于及时快速分析天然水中的类固醇污染物具有重要意义。
