Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
Anal Chem. 2024 May 28;96(21):8390-8398. doi: 10.1021/acs.analchem.3c05561. Epub 2024 May 8.
In this work, a microfluidic immunosensor chip was developed by incorporating microfluidic technology with electrochemiluminescence (ECL) for sensitive detection of human epidermal growth factor receptor-2 (HER2). The immunosensor chip can achieve robust reproducibility in mass production by integrating multiple detection units in a series. Notably, nanoscale materials can be better adapted to microfluidic systems, greatly enhancing the accuracy of the immunosensor chip. Ag@Au NCs closed by glutathione (GSH) were introduced in the ECL microfluidic immunosensor system with excellent and stable ECL performance. The synthesized CeO-Au was applied as a coreaction promoter in the ECL signal amplification system, which made the result of HER2 detection more reliable. In addition, the designed microfluidic immunosensor chip integrated the biosensing system into a microchip, realizing rapid and accurate detection of HER2 by its high throughput and low usage. The developed short peptide ligand NARKFKG (NRK) achieved an effective connection between the antibody and nanocarrier for improving the detection efficiency of the sensor. The immunosensor chip had better storage stability and sensitivity than traditional detection methods, with a wide detection range from 10 fg·mL to 100 ng·mL and a low detection limit (LOD) of 3.29 fg·mL. In general, a microfluidic immunosensor platform was successfully constructed, providing a new idea for breast cancer (BC) clinical detection.
在这项工作中，通过将微流控技术与电致化学发光（ECL）相结合，开发了一种用于灵敏检测人表皮生长因子受体-2（HER2）的微流控免疫传感器芯片。通过将多个检测单元集成在一个系列中，该免疫传感器芯片可以实现大规模生产的稳健可重复性。值得注意的是，纳米级材料可以更好地适应微流控系统，极大地提高了免疫传感器芯片的准确性。Ag@Au NCs 被谷胱甘肽（GSH）封闭，被引入到具有优异和稳定 ECL 性能的 ECL 微流控免疫传感器系统中。合成的 CeO-Au 被应用于 ECL 信号放大系统中的共反应促进剂，使 HER2 检测的结果更加可靠。此外，设计的微流控免疫传感器芯片将生物传感系统集成到微芯片中，通过高通量和低用量实现了 HER2 的快速准确检测。设计的短肽配体 NARKFKG（NRK）实现了抗体和纳米载体之间的有效连接，提高了传感器的检测效率。与传统检测方法相比，免疫传感器芯片具有更好的存储稳定性和灵敏度，检测范围从 10 fg·mL 到 100 ng·mL，检测限（LOD）低至 3.29 fg·mL。总之，成功构建了一种微流控免疫传感器平台，为乳腺癌（BC）临床检测提供了新的思路。
