Liu Ailing, Li Yuxia, Wang Qiqi, Zhang Xinrui, Xiong Jie, Li Yang, Lei Yonghui, Sun Yanfei
College of Life Sciences, Shihezi University, Shihezi, China.
Department of Plant Protection, College of Agriculture, Shihezi University, Shihezi, China.
Front Microbiol. 2022 Aug 15;13:971228. doi: 10.3389/fmicb.2022.971228. eCollection 2022.
Host plants influence rhizosphere microorganism composition through root secretions, and rhizosphere associated microorganisms influence Cistanche seeds germination. At present, little is known about effects of different host plants on soil bacteria and fungi in the rhizosphere of . High-throughput sequencing was used here to reveal the similarities and differences in the structural composition of the soil microbial community of from six host plants (i.e., , , , , , ). We discovered that -parasitizing (YRCR6) had the highest diversity of rhizosphere bacterial communities, and parasitizing (YRCR5) had the highest diversity of rhizosphere fungal communities. Fungal communities were more influenced by the host plant than bacterial communities. In addition, we discovered certain rhizosphere microorganisms that may be associated with Cistanche seeds germination, including , and , which are account for a relatively high proportion in , and parasitizing . Redundancy analysis results also revealed that AP, HCO , pH, Ca, SO , and K had a highly significant impact on the bacterial community structure ( < 0.01), while pH and SO had a significant impact on the fungal community structure ( < 0.05). Conclusively, differences were noted in the structure of rhizosphere bacterial and fungal communities of parasitizing different plants in the same habit and the difference may be related to the host plant. This result can provide a new ideas for the selection of host plants and the cultivation of .
寄主植物通过根系分泌物影响根际微生物组成，而根际相关微生物影响肉苁蓉种子萌发。目前，关于不同寄主植物对肉苁蓉根际土壤细菌和真菌的影响知之甚少。本研究利用高通量测序技术揭示了6种寄主植物（即[具体植物名称1]、[具体植物名称2]、[具体植物名称3]、[具体植物名称4]、[具体植物名称5]、[具体植物名称6]）根际土壤微生物群落结构组成的异同。我们发现，寄生在[寄主植物名称1]上的肉苁蓉（YRCR6）根际细菌群落多样性最高，寄生在[寄主植物名称2]上的肉苁蓉（YRCR5）根际真菌群落多样性最高。真菌群落比细菌群落更容易受到寄主植物的影响。此外，我们发现了一些可能与肉苁蓉种子萌发相关的根际微生物，包括[微生物名称1]、[微生物名称2]和[微生物名称3]，它们在寄生[寄主植物名称1]、[寄主植物名称2]和[寄主植物名称3]的肉苁蓉根际中所占比例相对较高。冗余分析结果还表明，有效磷（AP）、重碳酸盐（HCO₃⁻）、pH值、钙（Ca）、硫酸根（SO₄²⁻）和钾（K）对细菌群落结构有极显著影响（P < 0.01），而pH值和硫酸根对真菌群落结构有显著影响（P < 0.05）。总之，寄生在同一习性不同植物上的肉苁蓉根际细菌和真菌群落结构存在差异且这种差异可能与寄主植物有关。该结果可为寄主植物的选择和肉苁蓉的栽培提供新思路。
