Feng Zhan, Miao Yujing, Sun Xiao, Zheng Yan, Luo Guangming, Pei Jin, Huang Linfang
Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Front Microbiol. 2024 Jul 25;15:1279536. doi: 10.3389/fmicb.2024.1279536. eCollection 2024.
(C.A.Mey.) G. Beck is a perennial holoparasitic herb recognized for its medicinal properties, particularly in kidney-tonifying and laxative treatments. Despite its therapeutic potential, little is known about the endophyte communities inhabiting and its host plants, and how these microorganisms may impact the production and accumulation of metabolites in .
We conducted a dual analysis focusing on metabolomics of wild and microbiome characterization of both and its host plant, (Pall.) Moq. The metabolomics analysis revealed variations in metabolite composition across different parts of . Additionally, the microbiome analysis involved studying endophytic bacteria and fungi, comparing their community structures between parasitic and its host plant.
Significant variations in metabolite composition were observed through metabolomic profiling, which identified 93 secondary metabolites and 398 primary metabolites across various parts of . Emphasis was placed on differences in metabolite composition within the flowers. Microbiome analysis revealed differential community compositions of endophytic bacteria between the parasitic and host plants, whereas differences in endophytic fungi were less pronounced. Certain endophytes, such as Bacteroidota, Proteobacteria, Ascomycota, and Basidiomycota, were associated with the production of specific secondary metabolites in , including the plant-specific compound salsaside.
Our findings highlight the intricate relationship between and its endophytic microbiota, suggesting a potential role of these microorganisms in modulating the biosynthesis of bioactive compounds. The differential preferences of endophytic bacteria and fungi across various microenvironments within the parasitic plant system underscore the complexity of these interactions. Further elucidation of these dynamics could enhance our understanding of medicinal properties and its ecological adaptations as a holoparasitic herb.
（C.A. 梅伊）G. 贝克是一种多年生全寄生草本植物，以其药用特性而闻名，特别是在补肾和泻药治疗方面。尽管其具有治疗潜力，但对于栖息在其体内及其宿主植物中的内生菌群落，以及这些微生物如何影响其体内代谢物的产生和积累，人们知之甚少。
我们进行了一项双重分析，重点是野生G. 贝克的代谢组学以及其与宿主植物（Pall.）Moq. 的微生物组特征分析。代谢组学分析揭示了G. 贝克不同部位代谢物组成的差异。此外，微生物组分析包括研究内生细菌和真菌，比较寄生的G. 贝克与其宿主植物之间的群落结构。
通过代谢组学分析观察到代谢物组成存在显著差异，在G. 贝克的各个部位鉴定出93种次生代谢物和398种初级代谢物。重点关注了花内代谢物组成的差异。微生物组分析显示，寄生植物和宿主植物之间内生细菌的群落组成存在差异，而内生真菌的差异不太明显。某些内生菌，如拟杆菌门、变形菌门、子囊菌门和担子菌门，与G. 贝克中特定次生代谢物的产生有关，包括植物特有的化合物萨尔萨苷。
我们的研究结果突出了G. 贝克与其内生微生物群之间的复杂关系，表明这些微生物在调节生物活性化合物的生物合成中可能发挥作用。寄生植物系统内不同微环境中内生细菌和真菌的不同偏好强调了这些相互作用的复杂性。进一步阐明这些动态过程可以增强我们对G. 贝克作为全寄生草本植物的药用特性及其生态适应性的理解。
