Institute of Microbiology, University of Veterinary and Animal Sciences Lahore, 54600, Pakistan.
Department of Pathobiology, Faculty of Veterinary and Animal Sciences, MNS University of Agriculture, Multan 66000, Pakistan.
Biomed Res Int. 2022 Aug 21;2022:4539367. doi: 10.1155/2022/4539367. eCollection 2022.
is a major cause of gastroenteritis in humans. It has been reported that the pathogenesis of is closely related to the formation, adhesion, and invasion of flagella toxin in host epithelial cells. A putative transcriptional regulator, known as , is thought to be involved in the regulation of flagellar synthesis. However, confirmation of this hypothesis requires deep insight into the regulation mechanism of and its possible relationship with different antibiotics. Therefore, the study explained here was designed to determine the relationship and function (phenotypically and genotypically) of in the flagellar synthesis of NCTC11168. The study determined the mode of expression of and flagella-related genes under exposure to various drugs. To verify the involvement of protein in the metabolic pathway of thiamine, an enzymatic hydrolysis experiment was performed and analyzed through the application of mass spectrometry. The overexpression vector of NCTC11168 was also constructed to find out whether or not target genes were regulated by . The findings of the study showed that and other flagella-related genes were expressed differentially under the influence of various antibiotics including erythromycin, tylosin, azithromycin, gentamicin, etimicin, enrofloxacin, gatifloxacin, tetracycline, and tigecycline. The analysis showed that the protein did not catalyze the degradation of thiamine. In conclusion, the study aids in the understanding of the inter-relationship between the regulatory mechanism of flagella genes and the thiamine metabolic pathway.
是人类肠胃炎的主要病因。据报道，的发病机制与鞭毛毒素在宿主上皮细胞中的形成、黏附和侵袭密切相关。一种假定的转录调节剂，称为，被认为参与鞭毛合成的调节。然而，要证实这一假设，需要深入了解的调控机制及其与不同抗生素的可能关系。因此，本研究旨在确定在 NCTC11168 的鞭毛合成中与 flagella 相关的基因的关系和功能（表型和基因型）。研究确定了在暴露于各种药物时的表达模式和 flagella 相关基因。为了验证在硫胺素代谢途径中涉及的蛋白，进行了酶水解实验，并通过应用质谱进行了分析。还构建了 NCTC11168 的过表达载体，以确定靶基因是否由调节。研究结果表明，在各种抗生素如红霉素、泰乐菌素、阿奇霉素、庆大霉素、乙替米星、恩诺沙星、加替沙星、四环素和替加环素的影响下，和其他鞭毛相关基因的表达存在差异。分析表明，蛋白不催化硫胺素的降解。总之，该研究有助于理解鞭毛基因调控机制与硫胺素代谢途径之间的相互关系。
