Inter-Relationship Between a Transcriptional Regulator of Flagella Genes and Thiamine Metabolic Pathway in .

is a major cause of gastroenteritis in humans. It has been reported that the pathogenesis of is closely related to the formation, adhesion, and invasion of flagella toxin in host epithelial cells. A putative transcriptional regulator, known as , is thought to be involved in the regulation of flagellar synthesis. However, confirmation of this hypothesis requires deep insight into the regulation mechanism of and its possible relationship with different antibiotics. Therefore, the study explained here was designed to determine the relationship and function (phenotypically and genotypically) of in the flagellar synthesis of NCTC11168. The study determined the mode of expression of and flagella-related genes under exposure to various drugs. To verify the involvement of protein in the metabolic pathway of thiamine, an enzymatic hydrolysis experiment was performed and analyzed through the application of mass spectrometry. The overexpression vector of NCTC11168 was also constructed to find out whether or not target genes were regulated by . The findings of the study showed that and other flagella-related genes were expressed differentially under the influence of various antibiotics including erythromycin, tylosin, azithromycin, gentamicin, etimicin, enrofloxacin, gatifloxacin, tetracycline, and tigecycline. The analysis showed that the protein did not catalyze the degradation of thiamine. In conclusion, the study aids in the understanding of the inter-relationship between the regulatory mechanism of flagella genes and the thiamine metabolic pathway.