Jafari Fariba, Motavallihaghi Seyed Mousa, Bakhtiari Mehran, Maghsood Amir Hossein, Foroughi-Parvar Faeze
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Iran J Parasitol. 2022 Jan-Mar;17(1):36-42. doi: 10.18502/ijpa.v17i1.9014.
We aimed to evaluate contamination in conventional and industrial raw beef burger samples from butcheries and retail stores in Hamadan, western Iran.
Overall, 80 samples including 30 conventional and 50 industrial hamburgers were randomly obtained from different butcheries and supermarkets. All specimens were studied by digestion method following microscopic examination. Samples' genomic ribosomal DNA were amplified and nucleotide sequences were analyzed by BLAST for comparison with the sequences in the gene bank of the NCBI.
bradyzoites were detected in 46 of 80 (57.6%) samples. Positive specimens were included as 46 (57.6%) and 30 (37.5%) by digestion and molecular method, respectively. Differences between two studied (digestion and molecular) methods was statistically significant (=0.00). Twenty-six (86.5 %) of 30 conventional beef burgers and 20 (40%) of 50 industrial burgers were positive for sp. by digestion method. There was a significant difference between infested conventional and industrial beef burgers (=0.01).
The parasitic contamination of beef burgers implied a high level of infection in cattle. Felids as the definitive hosts for urged on the improvement of the hygienic conditions of keeping and feeding livestock in order to reduce the infection. Molecular techniques confirm species in meat products with high sensitivity and distinguish it from human species.
我们旨在评估伊朗西部哈马丹的肉店和零售店中传统和工业生产的生牛肉汉堡样本中的污染情况。
总共从不同的肉店和超市随机获取了80个样本，包括30个传统汉堡和50个工业汉堡。所有标本在显微镜检查后通过消化法进行研究。对样本的基因组核糖体DNA进行扩增，并通过BLAST分析核苷酸序列，以便与NCBI基因库中的序列进行比较。
在80个样本中的46个（57.6%）中检测到缓殖子。通过消化法和分子方法检测到的阳性标本分别为46个（57.6%）和30个（37.5%）。两种研究方法（消化法和分子法）之间的差异具有统计学意义（P = 0.00）。通过消化法，30个传统牛肉汉堡中有26个（86.5%）和50个工业汉堡中有20个（40%）对 种呈阳性。受感染的传统牛肉汉堡和工业牛肉汉堡之间存在显著差异（P = 0.01）。
牛肉汉堡的寄生虫污染表明牛的感染水平很高。猫科动物作为 的终末宿主，促使人们改善牲畜饲养和喂养的卫生条件，以减少感染。分子技术能够以高灵敏度确认肉类产品中的物种，并将其与人类物种区分开来。
