Rubiola S, Civera T, Ferroglio E, Zanet S, Zaccaria T, Brossa S, Cipriani R, Chiesa F
University of Turin, Department of Veterinary Science, 10095 Grugliasco, TO, Italy.
S.C. Microbiologia e Virologia U - AOU Città della Salute e della Scienza di Torino, Molinette, Italy.
Food Waterborne Parasitol. 2020 Feb 21;18:e00074. doi: 10.1016/j.fawpar.2020.e00074. eCollection 2020 Mar.
spp. are protozoan parasites which can infect a wide range of vertebrates, including humans; the latter can act as definitive hosts for two cattle spp.: and . Reports of intestinal sarcocystosis are well documented in the literature, but PCR-based methods have been scarcely used to identify species in human stools, and have been limited to the molecular analysis of 18S ribosomal RNA (18S rRNA) gene sequences. Since the mitochondrial cytochrome oxidase subunit I (COI) gene is one of the most promising tools for distinguishing between closely related spp., and taking into account the lack of publicly available COI sequences, in the present study we obtained the first partial COI sequence of from human stool samples of patient with gastrointestinal symptoms. We designed specific COI primers to develop a multiplex PCR method for the identification of spp. in cattle. The submission of the COI sequence described herein and the unambiguous identification of through the application of the new multiplex PCR is important for determining the prevalence of this zoonotic spp. in meat and the risk for consumers.
肉孢子虫属是原生动物寄生虫，可感染包括人类在内的多种脊椎动物；人类可作为两种牛肉孢子虫（牛犬肉孢子虫和人肉孢子虫）的终末宿主。肠道肉孢子虫病的报告在文献中有充分记载，但基于聚合酶链反应（PCR）的方法很少用于鉴定人类粪便中的肉孢子虫物种，并且仅限于对18S核糖体RNA（18S rRNA）基因序列进行分子分析。由于线粒体细胞色素c氧化酶亚基I（COI）基因是区分密切相关肉孢子虫物种最有前景的工具之一，并且考虑到缺乏公开可用的肉孢子虫COI序列，在本研究中，我们从有胃肠道症状患者的人类粪便样本中获得了首个肉孢子虫的部分COI序列。我们设计了特异性COI引物，以开发一种用于鉴定牛体内肉孢子虫物种的多重PCR方法。本文所述COI序列的提交以及通过应用新的多重PCR对肉孢子虫进行明确鉴定，对于确定这种人畜共患肉孢子虫物种在肉类中的流行情况以及对消费者的风险非常重要。
