Hillerdal M, Sperber G O, Bill A
Acta Physiol Scand. 1987 Jun;130(2):229-35. doi: 10.1111/j.1748-1716.1987.tb08132.x.
When the microsphere method of blood flow measurement is used in small organs, the number of spheres actually recovered may be small. This introduces errors which, however, are not necessarily important as compared with the biological variation. A mathematical model was constructed which allowed estimation of the extra uncertainty caused by the low number of spheres. It was applied to an experimental material of over 100 measurements of cochlear blood flow in the rat, where only about 60 spheres were recovered per cochlea. It was concluded that this paucity of spheres introduced only a small to moderate extra error. Simple approximate formulae were derived allowing easy estimation of the uncertainty caused by low number of spheres under different conditions. In general, there appear to be many situations where fewer microspheres than are commonly used would suffice.
当在小器官中使用微球法测量血流时，实际回收的微球数量可能很少。这会引入误差，然而，与生物学变异相比，这些误差不一定重要。构建了一个数学模型，该模型可以估计由于微球数量少而导致的额外不确定性。它被应用于对大鼠耳蜗血流进行的100多次测量的实验材料中，每个耳蜗仅回收约60个微球。得出的结论是，微球数量少仅引入了小到中等程度的额外误差。推导了简单的近似公式，便于估计在不同条件下微球数量少所导致的不确定性。一般来说，似乎在许多情况下，使用比常用数量更少的微球就足够了。
