Global Health Research Center, Duke Kunshan University, Kunshan, Jiangsu, China.
Division of Natural and Applied Sciences, Duke Kunshan University, Kunshan, Jiangsu, China.
Vet Med Sci. 2022 Sep;8(5):1982-1992. doi: 10.1002/vms3.869. Epub 2022 Sep 1.
Pigs are unique reservoirs for virus ecology. Despite the increased use of improved biosecurity measures, pig viruses readily circulate in Chinese swine farms.
The main objective of this study was to examine archived swine oral secretion samples with a panel of pan-species viral assays such that we might better describe the viral ecology of swine endemic viruses in Chinese farms.
Two hundred (n = 200) swine oral secretion samples, collected during 2015 and 2016 from healthy pigs on six swine farms in two provinces in China, were screened with molecular pan-species assays for coronaviruses (CoVs), adenoviruses (AdVs), enteroviruses (EVs), and paramyxoviruses (PMV). Samples were also screened for porcine circovirus (PCV) 3, porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV).
Among 200 swine oral secretion samples, 152 (76.0%) were found to have at least one viral detection. Thirty-four samples (17%) were positive for more than one virus, including 24 (70.5%) with dual detection and 10 (29.5%) with triple detection. Seventy-eight (39.0%) samples were positive for porcine AdVs, 22 (11.0%) were positive for porcine CoVs, 21 (10.5%) were positive for IAVs, 13 (6.5%) were positive for PCV, 7 (3.5%) were positive for PMV, six (3.0%) were positive for PRRSV and five (2.5%) were positive for porcine EV.
Our findings underscore the high prevalence of numerous viruses among production pigs in China and highlight the need for routine, periodic surveillance for novel virus emergence with the goal of protecting pigs.
猪是病毒生态学的独特宿主。尽管越来越多地使用改进的生物安全措施，但猪病毒仍在中国养猪场中广泛传播。
本研究的主要目的是利用泛种病毒检测试剂盒检查存档的猪口腔分泌样本，以便更好地描述中国猪场地方流行病毒的病毒生态学。
从中国两个省份的六家猪场的 2015 年和 2016 年的健康猪身上采集了 200 份猪口腔分泌样本，用分子泛种病毒检测试剂盒检测冠状病毒（CoVs）、腺病毒（AdVs）、肠道病毒（EVs）和副粘病毒（PMV）。还对猪圆环病毒 3 型（PCV3）、猪繁殖与呼吸综合征病毒（PRRSV）和甲型流感病毒（IAV）进行了检测。
在 200 份猪口腔分泌样本中，有 152 份（76.0%）至少检测到一种病毒。34 份样本（17%）检测到多种病毒，包括 24 份（70.5%）双重检测和 10 份（29.5%）三重检测。78 份（39.0%）样本检测到猪腺病毒阳性，22 份（11.0%）样本检测到猪冠状病毒阳性，21 份（10.5%）样本检测到甲型流感病毒阳性，13 份（6.5%）样本检测到猪圆环病毒阳性，7 份（3.5%）样本检测到副粘病毒阳性，6 份（3.0%）样本检测到猪繁殖与呼吸综合征病毒阳性，5 份（2.5%）样本检测到猪肠道病毒阳性。
我们的研究结果强调了中国生产猪中存在许多病毒的高流行率，并强调需要定期进行常规监测，以发现新出现的病毒，从而保护猪群。
