Centro de Investigación en Alimentación y Desarrollo, A. C. Avenida Sábalo-Cerritos S/N, Mazatlán, Sinaloa, México.
Consejo Nacional de Ciencia y Tecnología, Ciudad de México, México.
J Environ Sci Health B. 2022;57(10):775-785. doi: 10.1080/03601234.2022.2115780. Epub 2022 Sep 1.
Glyphosate [N-(phosphonomethyl)glycine] is one of the most popular herbicides worldwide. Globally, the use of glyphosate is increasing, and its residues have been found in drinking water and food products. The data regarding the possible toxic effects of this herbicide are controversial. Therefore, the aim of this study was to evaluate the effects of glyphosate at environmental concentrations in zebrafish () embryos. Embryos were exposed to 0, 1, 100, and 1,000 µg/L glyphosate for 96 h, and mortality, heart rate, and hatching rate were evaluated. After the experiment, RNA was extracted from the embryos for transcriptional analysis. No mortality was recorded, and exposure to 100 µg/L and 1,000 µg/L of glyphosate resulted in lower heart rates at 48 h. In addition, RNA-seq analysis revealed that glyphosate exposure induced subtle changes in gene transcription profiles. We found 30 differentially expressed genes; however, the highest glyphosate concentration (1,000 µg/L) induced the greatest number of differentially expressed genes involved in oocyte maturation, metabolic processes, histone deacetylation, and nervous system development.
草甘膦[N-（膦酸甲基）甘氨酸]是世界上最受欢迎的除草剂之一。全球范围内，草甘膦的使用量正在增加，其残留物已在饮用水和食品中被发现。关于这种除草剂可能产生的毒性影响的数据存在争议。因此，本研究旨在评估环境浓度的草甘膦对斑马鱼（）胚胎的影响。胚胎在 0、1、100 和 1000μg/L 的草甘膦中暴露 96 小时，评估死亡率、心率和孵化率。实验结束后，从胚胎中提取 RNA 进行转录分析。未记录到死亡率，暴露于 100μg/L 和 1000μg/L 的草甘膦会导致 48 小时时心率降低。此外，RNA-seq 分析显示，草甘膦暴露会引起基因转录谱的细微变化。我们发现了 30 个差异表达基因；然而，最高浓度的草甘膦（1000μg/L）诱导了最多数量的差异表达基因，这些基因涉及卵母细胞成熟、代谢过程、组蛋白去乙酰化和神经系统发育。
