College of Food Science and Nutritional Engineering (Institute of Nutrition and Health), China Agricultural University, Beijing, 100083, China.
Key Laboratory of Agricultural Genetically Modified Organisms Safety Evaluation (Edible), Ministry of Agriculture, Beijing, 100083, China.
Mikrochim Acta. 2022 Sep 1;189(9):365. doi: 10.1007/s00604-022-05459-3.
Since Salmonella can cause foodborne disease and public health safety issues and requires a robust, rapid, on-site detection method, a novel visual qualitative method with nano-gold-enhanced loop-mediated isothermal amplification (LAMP) reaction was established for detecting Salmonella in an integrated tube. During the experiment, nano-gold were used to enhance LAMP amplification, improving amplification efficiency and shortening the reaction time to within 30 min. Visual qualitative detection is achieved via negative staining, involving the addition of CuSO to the final products of the LAMP reaction. Ring-like white accumulation occurs in the absence of Salmonella targets but not when they are present. After completing the LAMP reaction, the integration tube was shaken gently for 1 min to observe the liquid phase system changes, realizing the closed tube detection of Salmonella. The process resolved the challenge presented by cross-contamination, false positives, and nonspecific amplification during the LAMP reaction. This method was used to detect Salmonella in milk, further highlighting its prospects in the field of rapid food safety detection.
由于沙门氏菌可引起食源性疾病和公共卫生安全问题，需要一种强大、快速、现场检测方法，因此建立了一种新颖的基于纳米金增强环介导等温扩增（LAMP）反应的可视化定性方法，用于在集成管中检测沙门氏菌。在实验中，纳米金用于增强 LAMP 扩增，提高了扩增效率，并将反应时间缩短至 30 分钟内。通过负染色进行可视化定性检测，即在 LAMP 反应的最终产物中加入 CuSO4。在不存在沙门氏菌靶标时会出现环状白色堆积，但存在靶标时则不会。完成 LAMP 反应后，轻轻摇动集成管 1 分钟，观察液相系统的变化，实现沙门氏菌的闭管检测。该过程解决了 LAMP 反应过程中交叉污染、假阳性和非特异性扩增带来的挑战。该方法用于检测牛奶中的沙门氏菌，进一步凸显了其在快速食品安全检测领域的应用前景。
