Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, PR China.
Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing 400044, PR China.
ACS Appl Mater Interfaces. 2022 Sep 14;14(36):41369-41378. doi: 10.1021/acsami.2c07660. Epub 2022 Sep 1.
Herein, we develop a hemin-functionalized microfluidic chip with dual-electric signal outputs for accurate determination of uric acid (UA). Hemin is designed as the catalyst, which could trigger a built-in reference signal. Carbon nanotube (CNT) and alkalinized titanium carbide (alk-TiCT) are used as attachment substrates to strengthen the signal. Benefiting from the synergistic action of hemin, CNT, and alk-TiCT, the hybrid functionalized sensor shows prominent electrochemical capacity, desirable catalytic activity, and unique built-in signal ability. Through density functional theory calculations, the structure-reactivity relationship and possible signal output mechanism are deeply investigated. The functionalized sensor is further integrated into a microfluidic chip to prepare a portable electrochemical sensing platform, in which multiple sample processing steps including primary filtration, target enrichment, and reliable analysis can be conducted step-by-step. Based on the abovementioned designs, the developed functionalized microfluidic platform presents desirable performance in UA determination with a detection limit of 0.41 μM. Furthermore, it is capable of accurately detecting UA in urine samples, providing a promising idea for biomolecule monitoring.
在这里，我们开发了一种具有双电信号输出的血红素功能化微流控芯片，用于准确测定尿酸（UA）。血红素被设计为催化剂，可以触发内置参考信号。碳纳米管（CNT）和堿化碳化钛（alk-TiCT）被用作附着基底，以增强信号。受益于血红素、CNT 和 alk-TiCT 的协同作用，混合功能化传感器表现出突出的电化学能力、理想的催化活性和独特的内置信号能力。通过密度泛函理论计算，深入研究了结构-反应性关系和可能的信号输出机制。该功能化传感器进一步集成到微流控芯片中，制备了一种便携式电化学传感平台，其中可以逐步进行包括初步过滤、目标富集和可靠分析在内的多个样品处理步骤。基于上述设计，开发的功能化微流控平台在 UA 测定中表现出良好的性能，检测限为 0.41 μM。此外，它能够准确检测尿液样本中的 UA，为生物分子监测提供了有前途的思路。
